Search

DJ-1 backbone assignment
Authors
Eliezer, D., Malgieri, G.
Assembly
DJ-1 protein
Entity
1. DJ-1 protein (polymer, Thiol state: all free), 197 monomers, 20955.92 Da Detail

MASKRALVIL AKGAEEMETV IPVDVMRRAG IKVTVAGLAG KDPVQCSRDV VICPDASLED AKKEGPYDVV VLPGGNLGAQ NLSESAAVKE ILKEQENRKG LIAAICAGPT ALLAHEIGFG SKVTTHPLAK DKMMNGGHYT YSENRVEKDG LILTSRGPGT SFEFALAIVE ALNGKEVAAQ VKAPLVLKDL EHHHHHH


Formula weight
20955.92 Da
Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.4 %, Completeness: 45.4 %, Completeness (bb): 77.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All45.4 % (998 of 2196)23.3 % (263 of 1130)65.0 % (560 of 861)85.4 % (175 of 205)
Backbone77.6 % (889 of 1146)46.8 % (180 of 385)93.2 % (534 of 573)93.1 % (175 of 188)
Sidechain22.5 % (277 of 1229)11.1 % (83 of 745)41.5 % (194 of 467) 0.0 % (0 of 17)
Aromatic 0.0 % (0 of 90) 0.0 % (0 of 45) 0.0 % (0 of 45)
Methyl20.7 % (53 of 256)12.5 % (16 of 128)28.9 % (37 of 128)

1. DJ-1

MASKRALVIL AKGAEEMETV IPVDVMRRAG IKVTVAGLAG KDPVQCSRDV VICPDASLED AKKEGPYDVV VLPGGNLGAQ NLSESAAVKE ILKEQENRKG LIAAICAGPT ALLAHEIGFG SKVTTHPLAK DKMMNGGHYT YSENRVEKDG LILTSRGPGT SFEFALAIVE ALNGKEVAAQ VKAPLVLKDL EHHHHHH

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6


#NameIsotope labelingTypeConcentration
1DJ-1[U-13C; U-15N; U-2H]0.3 mM
2DTTnatural abundance2 mM
3sodium phosphatenatural abundance20 mM
4H2Onatural abundance90 %
5D2Onatural abundance10 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6


#NameIsotope labelingTypeConcentration
6DJ-1[U-100% 15N]0.3 mM
7DTTnatural abundance2 mM
8sodium phosphatenatural abundance20 mM
9H2Onatural abundance90 %
10D2Onatural abundance10 %
Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6


#NameIsotope labelingTypeConcentration
11DJ-1[U-13C; U-15N]0.3 mM
12DTTnatural abundance2 mM
13sodium phosphatenatural abundance20 mM
14H2Onatural abundance90 %
15D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: 0.46 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.46 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.5 ppm, Outliers: 4 Detail
Release date
2011-03-13
Citation
Structural effects of Parkinson's disease linked DJ-1 mutations
Malgieri, G., Eliezer, D.
Protein Sci. (2008), 17, 855-868, PubMed 18436956 , DOI 10.1110/ps.073411608 ,
Related entities 1. DJ-1 protein, : 1 : 3 : 52 : 60 entities Detail
Interaction partners 1. DJ-1 protein, : 38 interactors Detail
Experiments performed 6 experiments Detail