BMRBj - BMREntryMaster

Found 1 Document (0.579 seconds)

BMRB: 17511

1DGC

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Leucine Zipper GCN4 coiled coil and x-form conformations.

Authors

Nikolaev, Y., Pervushin, K.

Assembly

LZ-GCN4 dimer

Entity

1. LZ-GCN4 dimer (polymer, Thiol state: not present), 36 monomers, 4294.928 × 3 Da Detail

GLQRMKQLED KVEELLSKNY HLENEVARLK KLVGER

Total weight

12884.785 Da

Max. entity weight

4294.928 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 97.2 %, Completeness: 29.4 %, Completeness (bb): 45.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	29.4 % (133 of 453)	26.7 % (65 of 243)	19.4 % (33 of 170)	87.5 % (35 of 40)
Backbone	45.8 % (99 of 216)	56.8 % (42 of 74)	20.8 % (22 of 106)	97.2 % (35 of 36)
Sidechain	15.1 % (41 of 271)	13.6 % (23 of 169)	18.4 % (18 of 98)	0.0 % (0 of 4)
Aromatic	0.0 % (0 of 12)	0.0 % (0 of 6)	0.0 % (0 of 6)
Methyl	33.3 % (14 of 42)	33.3 % (7 of 21)	33.3 % (7 of 21)

1. LZ-GCN4

GLQRMKQLED KVEELLSKNY HLENEVARLK KLVGER

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Temperature 310 K, pH 7.1

#	Name	Isotope labeling	Concentration
1	LZ-GCN4	[U-13C; U-15N]	0.3 mM
2	potassium chloride	natural abundance	80 mM
3	sodium acetate	natural abundance	40 mM

Chem. Shift Complete2

Sequence coverage: 97.2 %, Completeness: 33.9 %, Completeness (bb): 56.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.9 % (307 of 906)	31.7 % (154 of 486)	24.4 % (83 of 340)	87.5 % (70 of 80)
Backbone	56.9 % (246 of 432)	76.4 % (113 of 148)	29.7 % (63 of 212)	97.2 % (70 of 72)
Sidechain	13.1 % (71 of 542)	12.1 % (41 of 338)	15.3 % (30 of 196)	0.0 % (0 of 8)
Aromatic	0.0 % (0 of 24)	0.0 % (0 of 12)	0.0 % (0 of 12)
Methyl	26.2 % (22 of 84)	26.2 % (11 of 42)	26.2 % (11 of 42)

1. LZ-GCN4

GLQRMKQLED KVEELLSKNY HLENEVARLK KLVGER

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 3.2

#	Name	Isotope labeling	Concentration
1	LZ-GCN4	[U-13C; U-15N]	0.3 mM
2	potassium chloride	natural abundance	80 mM
3	sodium acetate	natural abundance	40 mM

Release date

2011-04-03

Citation

NMR spin state exchange spectroscopy reveals equilibrium of two distinct conformations of leucine zipper GCN4 in solution
Nikolaev, Y., Pervushin, K.
J. Am. Chem. Soc. (2007), 129, 6461-6469, PubMed 17469817 , DOI 10.1021/ja0685295 , Abstract

Related entities 1. LZ-GCN4 dimer, : 1 : 53 : 93 entities Detail

Interaction partners 1. LZ-GCN4 dimer, : 13 interactors Detail

More details for 13 interactors identified by 9 citations

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr17511_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr17511_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	LZ-GCN4_coiled_coil_shifts_at_pH_7.1	OK	70	97.2 (chain: 1, length: 36)
2	Assigned chemical shifts	LZ-GCN4_coiled_coil_and_x-form_shifts_at_pH_3.2	OK	276	97.2 (chain: 1, length: 36)
1	Chemical shift references	chemical_shift_reference_1	OK	3	No information

Assigned chemical shifts

Saveframe: LZ-GCN4_coiled_coil_shifts_at_pH_7.1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 36, Sequence coverage: 97.2%
- Total number of assignments
  - ¹H chemical shifts: 35
  - ¹⁵N chemical shifts: 35
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: LZ-GCN4_coiled_coil_and_x-form_shifts_at_pH_3.2
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 36, Sequence coverage: 97.2%
- Total number of assignments
  - ¹H chemical shifts: 138
  - ¹³C chemical shifts: 69
  - ¹⁵N chemical shifts: 69
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	243	35	14.4
¹⁵N chemical shifts	43	35	81.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	74	35	47.3
¹⁵N chemical shifts	36	35	97.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	169	0	0.0
¹⁵N chemical shifts	7	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	6	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	243	71	29.2
¹³C chemical shifts	170	35	20.6
¹⁵N chemical shifts	43	35	81.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	74	71	95.9
¹³C chemical shifts	72	35	48.6
¹⁵N chemical shifts	36	35	97.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	169	0	0.0
¹³C chemical shifts	98	0	0.0
¹⁵N chemical shifts	7	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	0	0.0
¹³C chemical shifts	22	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	6	0	0.0
¹³C chemical shifts	6	0	0.0

Keywords bzip, exchange, leucine zipper, NMR

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Found 1 Document (0.579 seconds)

BMRB: 17511

Sample

Sample

Related entities 1. LZ-GCN4 dimer, : 1 : 53 : 93 entities Detail

Interaction partners 1. LZ-GCN4 dimer, : 13 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail