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BMRB: 17550

2YHN

Backbone assignments of the IDOL RING domain

Authors

GOULT, B.T., Fairall, L., Tontonoz, P., Schwabe, J.JW.

Assembly

RING

Entity

1. RING (polymer, Thiol state: all free), 79 monomers, 8850.319 Da Detail

GAQQTRVLQE KLRKLKEAML CMVCCEEEIN STFCPCGHTV CCESCAAQLQ SCPVCRSRVE HVQHVYLPTH TSLLNLTVI

Formula weight

8850.319 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 45.7 %, Completeness (bb): 78.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.7 % (409 of 894)	24.0 % (111 of 463)	65.1 % (226 of 347)	85.7 % (72 of 84)
Backbone	78.4 % (367 of 468)	51.0 % (80 of 157)	91.9 % (216 of 235)	93.4 % (71 of 76)
Sidechain	22.7 % (114 of 503)	10.1 % (31 of 306)	43.4 % (82 of 189)	12.5 % (1 of 8)
Aromatic	0.0 % (0 of 34)	0.0 % (0 of 17)	0.0 % (0 of 17)
Methyl	22.9 % (22 of 96)	20.8 % (10 of 48)	25.0 % (12 of 48)

1. RING

GAQQTRVLQE KLRKLKEAML CMVCCEEEIN STFCPCGHTV CCESCAAQLQ SCPVCRSRVE HVQHVYLPTH TSLLNLTVI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	RING	[U-100% 13C; U-100% 15N]	0.6 (±0.05) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	100 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.55 ppm, Outliers: 2 Detail

Release date

2011-07-04

Citation

The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor
Zhang, L., Fairall, L., Goult, B.T., Calkin, A.C., Hong, C., Millard, C.J., Tontonoz, P., Schwabe, J.W.R.
Genes. Dev. (2011), 25, 1262-1274, PubMed 21685362 , DOI 10.1101/gad.2056211 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q8WY64 released on 2004-11-09
    Title MYLIP_HUMAN Entity E3 ubiquitin-protein ligase MYLIP
  Swiss-Prot: P51668 released on 1996-10-01
    Title UB2D1_HUMAN Entity Ubiquitin-conjugating enzyme E2 D1

Related entities 1. RING, : 1 : 1 : 6 : 13 entities Detail

Interaction partners 1. RING, : 7 interactors Detail

More details for 7 interactors identified by 4 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC