Backbone assignments of Anabaena Sensory Rhodopsin Transducer with D-tails
SLSIGRTCWA IAEGYIPPYG NGPEPQFISH ETVCILNAGD EDAHVEITIY YSDKEPVGPY RLTVPARRTK HVRFNDLNDP APIPHDTDFA SVIQSNVPIV VQHTRLDSRQ AENALLSTIA YANT
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 37.1 % (520 of 1400) | 19.7 % (142 of 721) | 52.9 % (293 of 554) | 68.0 % (85 of 125) |
Backbone | 65.4 % (472 of 722) | 41.2 % (100 of 243) | 78.4 % (287 of 366) | 75.2 % (85 of 113) |
Sidechain | 17.6 % (140 of 796) | 8.8 % (42 of 478) | 32.0 % (98 of 306) | 0.0 % (0 of 12) |
Aromatic | 1.8 % (2 of 110) | 1.8 % (1 of 55) | 1.9 % (1 of 54) | 0.0 % (0 of 1) |
Methyl | 11.5 % (17 of 148) | 10.8 % (8 of 74) | 12.2 % (9 of 74) |
1. ASRT
SLSIGRTCWA IAEGYIPPYG NGPEPQFISH ETVCILNAGD EDAHVEITIY YSDKEPVGPY RLTVPARRTK HVRFNDLNDP APIPHDTDFA SVIQSNVPIV VQHTRLDSRQ AENALLSTIA YANTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ASRT | [U-100% 13C; U-100% 15N; U-50% 2H] | 1 mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % | |
4 | Tris | natural abundance | 50 mM | |
5 | NaCl | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | ASRT | [U-100% 15N] | 1 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % | |
9 | Tris | natural abundance | 50 mM | |
10 | NaCl | natural abundance | 50 mM |