BMRB: 17592

Backbone assignments of Anabaena Sensory Rhodopsin Transducer with DNA

Authors

Wang, S., Kim, S., Jung, K., Ladizhansky, V., Brown, L.S.

Assembly

Anabaena Sensory Rhodopsin Transducer with DNA

Entity

1. ASRT (polymer, Thiol state: all free), 124 monomers, 13730.14 Da Detail

SLSIGRTCWA IAEGYIPPYG NGPEPQFISH ETVCILNAGD EDAHVEITIY YSDKEPVGPY RLTVPARRTK HVRFNDLNDP APIPHDTDFA SVIQSNVPIV VQHTRLDSRQ AENALLSTIA YANT

2. DNA (polymer, Thiol state: not present), 20 monomers, 6294.038 Da Detail

ATGACCTTTA GGAGGAAAGA

Total weight

20024.178 Da

Max. entity weight

13730.14 Da

Source organism

Trichormus variabilis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 74.3 %, Completeness: 33.1 %, Completeness (bb): 58.2 % Detail

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Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	1H	13C	15N
All	33.1 % (526 of 1588)	13.0 % (118 of 909)	56.3 % (312 of 554)	76.8 % (96 of 125)
Backbone	58.2 % (502 of 862)	26.1 % (100 of 383)	83.9 % (307 of 366)	84.1 % (95 of 113)
Sidechain	14.5 % (122 of 844)	3.4 % (18 of 526)	33.7 % (103 of 306)	8.3 % (1 of 12)
Aromatic	0.0 % (0 of 150)	0.0 % (0 of 95)	0.0 % (0 of 54)	0.0 % (0 of 1)
Methyl	9.9 % (15 of 152)	2.6 % (2 of 78)	17.6 % (13 of 74)

1. ASRT

SLSIGRTCWA IAEGYIPPYG NGPEPQFISH ETVCILNAGD EDAHVEITIY YSDKEPVGPY RLTVPARRTK HVRFNDLNDP APIPHDTDFA SVIQSNVPIV VQHTRLDSRQ AENALLSTIA YANT

2. DNA

ATGACCTTTA GGAGGAAAGA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	ASRT	[U-100% 13C; U-100% 15N; U-50% 2H]		1 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	Tris	natural abundance		50 mM
5	NaCl	natural abundance		50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
6	ASRT	[U-100% 15N]		1 mM
7	H20	natural abundance		90 %
8	D20	natural abundance		10 %
9	Tris	natural abundance		50 mM
10	NaCl	natural abundance		50 mM

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LACS Plot; CA

Referencing offset: 0.47 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.47 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 1.2 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Release date

2011-06-21

Citation

A eukaryotic-like interaction of soluble cyanobacterial sensory rhodopsin transducer with DNA
Wang, S., Kim, S., Jung, K., Ladizhansky, V., Brown, L.S.
J. Mol. Biol. (2011), 411, 449-462, PubMed 21683709 , DOI 10.1016/j.jmb.2011.06.002 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. ASRT, : 1 : 3 : 1 entities Detail

Experiments performed 8 experiments Detail

Chemical shift validation 4 contents Detail