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Solution Structure of a Minor and Transiently Formed State of a T4 Lysozyme Mutant
Authors
Bouvignies, G., Vallurupalli, P., Hansen, D., Correia, B., Lange, O., Bah, A., Vernon, R.M., Dahlquist, F.W., Baker, D., Kay, L.E.
Assembly
T4 L99A/G113A/R119P
Entity
1. T4 L99A/G113A/R119P (polymer, Thiol state: not present), 164 monomers, 18514.00 Da Detail

MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNTNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRAAAI NMVFQMGETG VAAFTNSLPM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL


Formula weight
18514.0 Da
Source organism
Escherichia virus T4
Exptl. method
SOLUTION NMR
Refine. method
distance geometry, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 55.5 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All55.5 % (1079 of 1945)41.4 % (416 of 1006)67.3 % (503 of 747)83.3 % (160 of 192)
Backbone98.1 % (948 of 966)97.5 % (316 of 324)98.1 % (473 of 482)99.4 % (159 of 160)
Sidechain25.1 % (284 of 1133)14.7 % (100 of 682)43.7 % (183 of 419) 3.1 % (1 of 32)
Aromatic 0.0 % (0 of 138) 0.0 % (0 of 69) 0.0 % (0 of 66) 0.0 % (0 of 3)
Methyl19.4 % (38 of 196)13.3 % (13 of 98)25.5 % (25 of 98)

1. T4 L99A/G113A/R119P

MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNTNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRAAAI NMVFQMGETG VAAFTNSLPM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 307 K, pH 5.5


#NameIsotope labelingTypeConcentration
1T4 L99A/G113A/R119P[U-100% 13C; U-100% 15N]1.5 mM
2H2Onatural abundance90 %
3D2Onatural abundance10 %
4NaClnatural abundance25 mM
5EDTAnatural abundance2 mM
6NaN3natural abundance2 mM
7sodium phosphatenatural abundance50 mM

LACS Plot; CA
Referencing offset: -0.32 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.32 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.03 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.31 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 2LC9, Strand ID: A Detail


Release date
2011-05-31
Citation
Solution structure of a minor and transiently formed state of a T4 lysozyme mutant
Bouvignies, G., Vallurupalli, P., Hansen, D., Correia, B.E., Lange, O., Bah, A., Vernon, R.M., Dahlquist, F.W., Baker, D., Kay, L.E.
Nature (2011), 477, 111-114, PubMed 21857680 , DOI 10.1038/nature10349 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 17604 released on 2011-05-31
    Title Solution Structure of a Minor and Transiently Formed State of a T4 Lysozyme Mutant
Related entities 1. T4 L99A/G113A/R119P, : 1 : 78 : 31 entities Detail
Experiments performed 8 experiments Detail
Keywords T4 Lysozyme L99A/G113A/R119P