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Solution Structure of a Minor and Transiently Formed State of a T4 Lysozyme Mutant
Authors
Bouvignies, G., Vallurupalli, P., Hansen, D., Correia, B., Lange, O., Bah, A., Vernon, R.M., Dahlquist, F.W., Baker, D., Kay, L.E.
Assembly
T4 L99A
Entity
1. T4 L99A (polymer, Thiol state: not present), 164 monomers, 18559.05 Da Detail

MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNTNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRAAAI NMVFQMGETG VAGFTNSLRM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL


Formula weight
18559.05 Da
Source organism
Escherichia virus T4
Exptl. method
SOLUTION NMR
Refine. method
distance geometry, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 35.9 %, Completeness (bb): 69.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All35.9 % (700 of 1948)27.4 % (276 of 1008)35.7 % (266 of 746)81.4 % (158 of 194)
Backbone69.6 % (673 of 967)78.5 % (255 of 325)54.1 % (260 of 481)98.1 % (158 of 161)
Sidechain 3.2 % (36 of 1134) 3.2 % (22 of 683) 3.3 % (14 of 418) 0.0 % (0 of 33)
Aromatic 0.0 % (0 of 138) 0.0 % (0 of 69) 0.0 % (0 of 66) 0.0 % (0 of 3)
Methyl 4.6 % (9 of 194) 4.1 % (4 of 97) 5.2 % (5 of 97)

1. T4 L99A

MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI GRNTNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRAAAI NMVFQMGETG VAGFTNSLRM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI TTFRTGTWDA YKNL

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5


#NameIsotope labelingTypeConcentration
1T4 L99A[U-15N; U-2H]1.5 mM
2H2Onatural abundance90 %
3D2Onatural abundance10 %
4NaClnatural abundance25 mM
5EDTAnatural abundance2 mM
6NaN3natural abundance2 mM
7sodium phosphatenatural abundance50 mM
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5


#NameIsotope labelingTypeConcentration
8T4 L99A[U-13C; U-15N; U-2H]1.5 mM
9H2Onatural abundance90 %
10D2Onatural abundance10 %
11NaClnatural abundance25 mM
12EDTAnatural abundance2 mM
13NaN3natural abundance2 mM
14sodium phosphatenatural abundance50 mM
Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5


#NameIsotope labelingTypeConcentration
15T4 L99A[U-13Ca; U-15N]1.5 mM
16D2Onatural abundance100 %
17NaClnatural abundance25 mM
18EDTAnatural abundance2 mM
19NaN3natural abundance2 mM
20sodium phosphatenatural abundance50 mM
Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5


#NameIsotope labelingTypeConcentration
21T4 L99A[U-13C; U-15N; U-50% 2H]1.5 mM
22D2Onatural abundance100 %
23NaClnatural abundance25 mM
24EDTAnatural abundance2 mM
25NaN3natural abundance2 mM
26sodium phosphatenatural abundance50 mM
Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5


#NameIsotope labelingTypeConcentration
27T4 L99A[ U-15N] 13CH3 Met1.5 mM
28D2Onatural abundance100 %
29NaClnatural abundance25 mM
30EDTAnatural abundance2 mM
31NaN3natural abundance2 mM
32sodium phosphatenatural abundance50 mM

Protein Blocks Logo
Calculated from 10 models in PDB: 2LCB, Strand ID: A Detail


Release date
2011-05-31
Citation
Solution structure of a minor and transiently formed state of a T4 lysozyme mutant
Bouvignies, G., Vallurupalli, P., Hansen, D., Correia, B.E., Lange, O., Bah, A., Vernon, R.M., Dahlquist, F.W., Baker, D., Kay, L.E.
Nature (2011), 477, 111-114, PubMed 21857680 , DOI 10.1038/nature10349 ,
Related entities 1. T4 L99A, : 1 : 34 : 40 : 35 entities Detail
Experiments performed 29 experiments Detail
Keywords Excited State, T4 Lysozyme L99A