BMRB: 17610

Solution structure of GppNHp-bound H-RasT35S mutant protein.

Authors

Araki, M.

Assembly

GppNHp-bound H-RasT35S mutant protein

Entity

1. GppNHp-bound H-RasT35S mutant protein (polymer), 172 monomers, 19365.50 Da Detail

GPLGSDMTEY KLVVVGAGGV GKSALTIQLI QNHFVDEYDP SIEDSYRKQV VIDGETCLLD ILDTAGQEEY SAMRDQYMRT GEGFLCVFAI NNTKSFEDIH QYREQIKRVK DSDDVPMVLV GNKCDLAART VESRQAQDLA RSYGIPYIET SAKTRQGVED AFYTLVREIR QH

2. PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER (non-polymer, Thiol state: not present), 522.196 Da
3. MG (non-polymer, Thiol state: not present), 24.305 Da

Total weight

19912.0 Da

Max. entity weight

19365.5 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 95.1 %, Completeness (bb): 96.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.1 % (1872 of 1968)	95.4 % (973 of 1020)	94.4 % (722 of 765)	96.7 % (177 of 183)
Backbone	96.2 % (985 of 1024)	97.2 % (343 of 353)	95.4 % (480 of 503)	96.4 % (162 of 168)
Sidechain	94.4 % (1041 of 1103)	94.5 % (630 of 667)	94.1 % (396 of 421)	100.0 % (15 of 15)
Aromatic	90.3 % (121 of 134)	95.5 % (64 of 67)	85.1 % (57 of 67)
Methyl	97.9 % (190 of 194)	97.9 % (95 of 97)	97.9 % (95 of 97)

1. GppNHp-bound H-RasT35S mutant protein

GPLGSDMTEY KLVVVGAGGV GKSALTIQLI QNHFVDEYDP SIEDSYRKQV VIDGETCLLD ILDTAGQEEY SAMRDQYMRT GEGFLCVFAI NNTKSFEDIH QYREQIKRVK DSDDVPMVLV GNKCDLAART VESRQAQDLA RSYGIPYIET SAKTRQGVED AFYTLVREIR QH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	GppNHp-bound H-RasT35S mutant protein	[U-98% 13C; U-98% 15N]		1.0 ~ 2.0 mM
2	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	natural abundance		1.0 ~ 2.0 mM
3	magnesium ions	natural abundance		10 mM
4	sodium chloride	natural abundance		150 mM
5	sodium phosphate	natural abundance		25 mM
6	H20	natural abundance		90 %
7	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
8	GppNHp-bound H-RasT35S mutant protein	[U-98% 13C; U-98% 15N]		1.0 ~ 2.0 mM
9	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	natural abundance		1.0 ~ 2.0 mM
10	magnesium ions	natural abundance		10 mM
11	sodium chloride	natural abundance		150 mM
12	sodium phosphate	natural abundance		25 mM
13	H20	natural abundance		90 %
14	D2O	natural abundance		10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2LCF, Strand ID: A Detail

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Heteronucl. T₁

130 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 749.93 MHz, Pressure 1 atm, Temperature 298 K, pH 6.8 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.8

Experiment name 2D 1H-15N HSQC

Heteronucl. T₂

130 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 749.93 MHz, Pressure 1 atm, Temperature 298 K, pH 6.8 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.8

Experiment name 2D 1H-15N HSQC

Heteronucl. NOE

130 NOE values in 1 lists
Value type peak height, Field strength (¹H) 749.93 MHz, Pressure 1 atm, Temperature 298 K, pH 6.8 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.8

Experiment name 2D 1H-15N HSQC

Heteronucl. T₁/T₂

130 T₁/T₂ values in 1 lists
Field strength (¹H) 749.93 MHz, Pressure 1 atm, Temperature 298 K, pH 6.8 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.8

Release date

2011-05-09

Citation

Solution structure of the state 1 conformer of GTP-bound H-Ras protein and distinct dynamic properties between the state 1 and state 2 conformers
Araki, M., Shima, F., Yoshikawa, Y., Muraoka, S., Ijiri, Y., Nagahara, Y., Shirono, T., Kataoka, T., Tamura, A.
J. Biol. Chem. (2011), 286, 39644-39653, PubMed 21930707 , DOI 10.1074/jbc.M111.227074 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. GppNHp-bound H-RasT35S mutant protein, : 1 : 5 : 1 : 2 : 291 entities Detail

Interaction partners 1. GppNHp-bound H-RasT35S mutant protein, : 60 interactors Detail

Experiments performed 13 experiments Detail