BMRBj - BMREntryMaster

Found 1 Document (0.676 seconds)

BMRB: 17705

2LEA

na1

Authors

Daubner, G.

Assembly

SRSF2 RRM

Entity

1. SRSF2 RRM (polymer, Thiol state: not present), 135 monomers, 15227.82 Da Detail

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMSYGRP PPDVEGMTSL KVDNLTYRTS PDTLRRVFEK YGRVGDVYIP RDRYTKESRG FAFVRFHDKR DAEDAMDAMD GAVLDGRELR VQMARYGRPP DSHHS

Formula weight

15227.82 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 74.8 %, Completeness: 62.3 %, Completeness (bb): 58.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	62.3 % (939 of 1507)	70.9 % (561 of 791)	48.0 % (281 of 585)	74.0 % (97 of 131)
Backbone	58.3 % (463 of 794)	70.8 % (196 of 277)	44.1 % (172 of 390)	74.8 % (95 of 127)
Sidechain	67.0 % (558 of 833)	71.0 % (365 of 514)	60.6 % (191 of 315)	50.0 % (2 of 4)
Aromatic	57.8 % (74 of 128)	76.6 % (49 of 64)	39.1 % (25 of 64)
Methyl	86.7 % (78 of 90)	86.7 % (39 of 45)	86.7 % (39 of 45)

1. SRSF2 RRM

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMSYGRP PPDVEGMTSL KVDNLTYRTS PDTLRRVFEK YGRVGDVYIP RDRYTKESRG FAFVRFHDKR DAEDAMDAMD GAVLDGRELR VQMARYGRPP DSHHS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
8	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
9	L-Arg	natural abundance	50 mM
10	L-Glu	natural abundance	50 mM
11	NaH2PO4	natural abundance	20 mM
12	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.53 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.53 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LEA, Strand ID: A Detail

Release date

2011-10-25

Citation

A syn-anti conformational difference allows SRSF2 to recognize guanines and cytosines equally well
Daubner, G.M., Clery, A., Jayne, S., Stevenin, J., Allain, F.
EMBO J. (2012), 31, 162-174, PubMed 22002536 , DOI 10.1038/emboj.2011.367 , Abstract

Related entities 1. SRSF2 RRM, : 1 : 2 : 14 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

3 2D 1H-1H TOCSY

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
8	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
9	L-Arg	natural abundance	50 mM
10	L-Glu	natural abundance	50 mM
11	NaH2PO4	natural abundance	20 mM
12	D2O	natural abundance	100 %

4 3D HCACO

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

5 3D HNCO

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

6 3D HNCA

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

7 3D HN(CO)CA

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

8 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

9 3D HNCACB

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

10 3D H(CCO)NH

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

11 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

12 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

13 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM 2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

14 2D 1H-1H NOESY

Instrument

Bruker Avance - 700 MHz cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
8	SRSF2 RRM 1	[U-15N]	0.75 (±0.1) mM
9	L-Arg	natural abundance	50 mM
10	L-Glu	natural abundance	50 mM
11	NaH2PO4	natural abundance	20 mM
12	D2O	natural abundance	100 %

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_17705_2lea.nef
Input source #2: Coordindates	2lea.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

------------------------------------------10--------20--------30--------40--------50--------60------
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMSYGRPPPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKR
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMSYGRPPPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKR
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

--70--------80--------90-------100-
DAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHS
|||||||||||||||||||||||||||||||||||
DAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHS
-------110-------120-------130-----

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	135	0	0	100.0

Content subtype: combined_17705_2lea.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	950		72.6 (chain: A, length: 135)
1	Distance restraints	AMBER_distance_constraints_2	OK	4487 (1)	noe	72.6 (chain: A, length: 135)
2	Distance restraints	AMBER_distance_constraints_3	Warning	54 (1)	hbond	25.9 (chain: A, length: 135)
1	Dihedral angle restraints	AMBER_dihedral_5	Warning	1 (1)	.	1.5 (chain: A, length: 135)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 135, Sequence coverage: 72.6%
- Total number of assignments
  - ¹H chemical shifts: 590
  - ¹³C chemical shifts: 264
  - ¹⁵N chemical shifts: 96
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	791	556	70.3
¹³C chemical shifts	585	264	45.1
¹⁵N chemical shifts	147	96	65.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	277	193	69.7
¹³C chemical shifts	270	76	28.1
¹⁵N chemical shifts	127	91	71.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	514	363	70.6
¹³C chemical shifts	315	188	59.7
¹⁵N chemical shifts	20	5	25.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	54	43	79.6
¹³C chemical shifts	54	43	79.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	64	49	76.6
¹³C chemical shifts	64	25	39.1

Distance restraints

Saveframe: AMBER_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 135, Sequence coverage: 72.6%
- Total number of restraints: 4450
- Weight of restraints: 1.0 (4450)
- Potential type of restraints: square-well-parabolic (4450)
- Range of distance target values: 2.37, 5.6 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: AMBER_distance_constraints_3
  - Status: Warning
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 135, Sequence coverage: 25.9%
  - Total number of restraints: 54
  - Weight of restraints: 1.0 (54)
  - Potential type of restraints: square-well-parabolic (54)
  - Range of distance target values: 2.35, 3.15 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: AMBER_dihedral_5
- Status: Warning
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 135, Sequence coverage: 1.5%
- Total number of restraints: 1
- Total number of restraints per polymer type: 1
- Weight of restraints: 1.0 (1)
- Potential type of restraints: square-well-parabolic (1)
- Dihedral angle restraints per residue

Keywords RNA binding Protein, RRM, splicing factor, SR protein

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.676 seconds)

BMRB: 17705

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. SRSF2 RRM, : 1 : 2 : 14 entities Detail

Experiments performed 14 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 5 contents Detail