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BMRB: 17706

2LEB

na2

Authors

Daubner, G.

Assembly

SRSF2

Entity

1. SRSF2 RRM (polymer, Thiol state: not present), 135 monomers, 15227.82 Da Detail

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMSYGRP PPDVEGMTSL KVDNLTYRTS PDTLRRVFEK YGRVGDVYIP RDRYTKESRG FAFVRFHDKR DAEDAMDAMD GAVLDGRELR VQMARYGRPP DSHHS

2. RNA (polymer, Thiol state: not present), 6 monomers, 1915.122 Da Detail

UCCAGU

Total weight

17142.941 Da

Max. entity weight

15227.82 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 74.5 %, Completeness: 59.9 %, Completeness (bb): 54.3 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	59.9 % (961 of 1605)	70.7 % (597 of 845)	43.5 % (272 of 626)	68.7 % (92 of 134)
Backbone	54.3 % (467 of 860)	68.4 % (214 of 313)	38.8 % (163 of 420)	70.9 % (90 of 127)
Sidechain	66.0 % (571 of 865)	72.0 % (383 of 532)	57.1 % (186 of 326)	28.6 % (2 of 7)
Aromatic	56.4 % (88 of 156)	76.9 % (60 of 78)	37.3 % (28 of 75)	0.0 % (0 of 3)
Methyl	81.1 % (73 of 90)	80.0 % (36 of 45)	82.2 % (37 of 45)

1. SRSF2 RRM

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMSYGRP PPDVEGMTSL KVDNLTYRTS PDTLRRVFEK YGRVGDVYIP RDRYTKESRG FAFVRFHDKR DAEDAMDAMD GAVLDGRELR VQMARYGRPP DSHHS

2. RNA

UCCAGU

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM + UCCAGU1	[U-15N]	0.75 (±0.1) mM
2	SRSF2 RRM + UCCAGU2	[U-13C; U-15N]	0.75 (±0.1) mM
3	L-Arg	natural abundance	50 mM
4	L-Glu	natural abundance	50 mM
5	NaH2PO4	natural abundance	20 mM
6	D2O	natural abundance	10 %
7	H2O	natural abundance	90 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
8	SRSF2 RRM + UCCAGU1	[U-15N]	0.75 (±0.1) mM
9	L-Arg	natural abundance	50 mM
10	L-Glu	natural abundance	50 mM
11	NaH2PO4	natural abundance	20 mM
12	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.66 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.66 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LEB, Strand ID: A Detail

Release date

2011-10-25

Citation

A syn-anti conformational difference allows SRSF2 to recognize guanines and cytosines equally well
Daubner, G.M., Clery, A., Jayne, S., Stevenin, J., Allain, F.
EMBO J. (2012), 31, 162-174, PubMed 22002536 , DOI 10.1038/emboj.2011.367 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 17705 released on 2011-10-25
    Title na1
  BMRB: 17707 released on 2011-10-25
    Title na3

Related entities 1. SRSF2 RRM, : 1 : 2 : 14 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC