BMRBj - BMREntryMaster

Found 1 Document (0.872 seconds)

BMRB: 17707

2LEC

na3

Authors

Daubner, G.

Assembly

SRSF2 RRM/RNA complex

Entity

1. SRSF2 RRM (polymer, Thiol state: not present), 135 monomers, 15227.82 Da Detail

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMSYGRP PPDVEGMTSL KVDNLTYRTS PDTLRRVFEK YGRVGDVYIP RDRYTKESRG FAFVRFHDKR DAEDAMDAMD GAVLDGRELR VQMARYGRPP DSHHS

2. RNA (5'-R(*UP*GP*GP*AP*GP*U)-3') (polymer, Thiol state: not present), 6 monomers, 1995.170 Da Detail

UGGAGU

Total weight

17222.99 Da

Max. entity weight

15227.82 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 77.3 %, Completeness: 62.4 %, Completeness (bb): 58.0 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	62.4 % (999 of 1601)	72.1 % (606 of 841)	48.1 % (300 of 624)	68.4 % (93 of 136)
Backbone	58.0 % (499 of 860)	71.9 % (225 of 313)	43.8 % (184 of 420)	70.9 % (90 of 127)
Sidechain	66.8 % (575 of 861)	71.8 % (379 of 528)	59.9 % (194 of 324)	22.2 % (2 of 9)
Aromatic	56.4 % (88 of 156)	74.4 % (58 of 78)	41.1 % (30 of 73)	0.0 % (0 of 5)
Methyl	88.9 % (80 of 90)	88.9 % (40 of 45)	88.9 % (40 of 45)

1. SRSF2 RRM

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMSYGRP PPDVEGMTSL KVDNLTYRTS PDTLRRVFEK YGRVGDVYIP RDRYTKESRG FAFVRFHDKR DAEDAMDAMD GAVLDGRELR VQMARYGRPP DSHHS

2. RNA (5'-R(*UP*GP*GP*AP*GP*U)-3')

UGGAGU

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
1	SRSF2 RRM	[U-15N]	0.75 (±0.1) mM
2	UGGAGU1	[U-15N]	0.75 (±0.1) mM
3	SRSF2 RRM	[U-13C; U-15N]	0.75 (±0.1) mM
4	UGGAGU2	[U-13C; U-15N]	0.75 (±0.1) mM
5	L-Arg	natural abundance	50 mM
6	L-Glu	natural abundance	50 mM
7	NaH2PO4	natural abundance	20 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 310.8 K, pH 5.5, Details 50mM L-Arg; 50mM L-Glu; 20mM NaH2PO4; ph 5.5

#	Name	Isotope labeling	Concentration
10	SRSF2 RRM	[U-15N]	0.75 (±0.1) mM
11	UGGAGU1	[U-15N]	0.75 (±0.1) mM
12	L-Arg	natural abundance	50 mM
13	L-Glu	natural abundance	50 mM
14	NaH2PO4	natural abundance	20 mM
15	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.57 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.57 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LEC, Strand ID: A Detail

Release date

2011-10-25

Citation

A syn-anti conformational difference allows SRSF2 to recognize guanines and cytosines equally well
Daubner, G.M., Clery, A., Jayne, S., Stevenin, J., Allain, F.
EMBO J. (2012), 31, 162-174, PubMed 22002536 , DOI 10.1038/emboj.2011.367 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 17705 released on 2011-10-25
    Title na1
  BMRB: 17706 released on 2011-10-25
    Title na2

Related entities 1. SRSF2 RRM, : 1 : 2 : 14 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC