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BMRB: 17714

2LEJ

human prion protein mutant HuPrP(90-231, M129, V210I)

Authors

Biljan, I., Ilc, G., Giachin, G., Raspadori, A., Zhukov, I., Plavec, J., Legname, G.

Assembly

human prion protein mutant HuPrP(90-231, M129, V210I)

Entity

1. human prion protein mutant HuPrP(90-231, M129, V210I) (polymer, Thiol state: all disulfide bound), 147 monomers, 16633.37 Da Detail

GAMDPGQGGG THSQWNKPSK PKTNMKHMAG AAAAGAVVGG LGGYMLGSAM SRPIIHFGSD YEDRYYRENM HRYPNQVYYR PMDEYSNQNN FVHDCVNITI KQHTVTTTTK GENFTETDVK MMERVIEQMC ITQYERESQA YYQRGSS

Formula weight

16633.37 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS95:SG	1:CYS130:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 86.7 %, Completeness (bb): 81.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.7 % (1452 of 1675)	92.9 % (813 of 875)	75.9 % (486 of 640)	95.6 % (153 of 160)
Backbone	81.3 % (707 of 870)	94.4 % (286 of 303)	66.9 % (285 of 426)	96.5 % (136 of 141)
Sidechain	92.8 % (870 of 937)	92.1 % (527 of 572)	94.2 % (326 of 346)	89.5 % (17 of 19)
Aromatic	87.0 % (134 of 154)	87.0 % (67 of 77)	86.8 % (66 of 76)	100.0 % (1 of 1)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. HuPrP

GAMDPGQGGG THSQWNKPSK PKTNMKHMAG AAAAGAVVGG LGGYMLGSAM SRPIIHFGSD YEDRYYRENM HRYPNQVYYR PMDEYSNQNN FVHDCVNITI KQHTVTTTTK GENFTETDVK MMERVIEQMC ITQYERESQA YYQRGSS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 20 mM sodium acetic buffer, pH 5.5

#	Name	Isotope labeling	Concentration
1	HuPrP	[U-100% 13C; U-100% 15N]	0.6 mM
2	sodium acetate	natural abundance	20 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 20 mM sodium acetic buffer, pD 5.9

#	Name	Isotope labeling	Concentration
5	HuPrP	[U-100% 13C; U-100% 15N]	0.6 mM
6	sodium acetate	natural abundance	20 mM
7	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LEJ, Strand ID: A Detail

Release date

2011-08-18

Citation

Toward the molecular basis of inherited prion diseases: NMR structure of the human prion protein with V210I mutation
Biljan, I., Ilc, G., Giachin, G., Raspadori, A., Zhukov, I., Plavec, J., Legname, G.
J. Mol. Biol. (2011), 412, 660-673, PubMed 21839748 , DOI 10.1016/j.jmb.2011.07.067 , Abstract

Related entities 1. human prion protein mutant HuPrP(90-231, M129, V210I), : 1 : 1 : 99 entities Detail

Interaction partners 1. human prion protein mutant HuPrP(90-231, M129, V210I), : 73 interactors Detail

More details for 73 interactors identified by 15 citations