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Structure of a monomeric mutant of the HIV-1 capsid protein
Authors
Tzou, Y., Shin, R.S., Krishna, N.
Assembly
HIV-1 CA
Entity
1. HIV-1 CA (polymer, Thiol state: all free), 240 monomers, 26609.18 Da Detail

MPIVQNLQGQ MVHQAISPRT LNAWVKVVEE KAFSPEVIPM FSALSEGATP QDLNTMLNTV GGHQAAMQML KETINEEAAE WDRLHPVHAG PIAPGQMREP RGSDIAGTTS TLQEQIGWMT HNPPIPVGEI YKRWIILGLN KIVRMYSPTS ILDIRQGPKE PFRDYVDRFY KTLRAEQASQ EVKNAATETL LVQNANPDCK TILKALGPGA TLEEMMTACQ GVGGPGHKAR VLVEHHHHHH


Formula weight
26609.18 Da
Source organism
Human immunodeficiency virus
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.1 %, Completeness: 84.9 %, Completeness (bb): 93.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All84.9 % (2361 of 2781)84.5 % (1220 of 1444)85.4 % (918 of 1075)85.1 % (223 of 262)
Backbone93.6 % (1297 of 1386)94.6 % (437 of 462)91.9 % (645 of 702)96.8 % (215 of 222)
Sidechain78.6 % (1271 of 1617)79.7 % (783 of 982)80.7 % (480 of 595)20.0 % (8 of 40)
Aromatic24.4 % (41 of 168)44.0 % (37 of 84) 2.5 % (2 of 80)50.0 % (2 of 4)
Methyl99.3 % (268 of 270)99.3 % (134 of 135)99.3 % (134 of 135)

1. HIV-1 CA

MPIVQNLQGQ MVHQAISPRT LNAWVKVVEE KAFSPEVIPM FSALSEGATP QDLNTMLNTV GGHQAAMQML KETINEEAAE WDRLHPVHAG PIAPGQMREP RGSDIAGTTS TLQEQIGWMT HNPPIPVGEI YKRWIILGLN KIVRMYSPTS ILDIRQGPKE PFRDYVDRFY KTLRAEQASQ EVKNAATETL LVQNANPDCK TILKALGPGA TLEEMMTACQ GVGGPGHKAR VLVEHHHHHH

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.5, Details 15N-labeled HIV-1 CA DM


#NameIsotope labelingTypeConcentration
1HIV-1 CA[U-98% 15N]2 mM
2sodium chloridenatural abundance25 mM
3sodium acetate[U-99% 2H]25 mM
4DTT[U-99% 2H]10 mM
5sodium azidenatural abundance0.02 %
6AEBSF protease inhibitornatural abundance0.1 mM
7EDTA[U-99% 2H]1 mM
8H2Onatural abundance90 %
9D2Onatural abundance10 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.5, Details 13C / 15N-labeled HIV-1 CA DM


#NameIsotope labelingTypeConcentration
10HIV-1 CA[U-98% 13C; U-98% 15N]2 mM
11sodium chloridenatural abundance25 mM
12sodium acetate[U-99% 2H]25 mM
13DTT[U-99% 2H]10 mM
14sodium azidenatural abundance0.02 %
15AEBSF protease inhibitornatural abundance0.1 mM
16EDTA[U-99% 2H]1 mM
17H2Onatural abundance90 %
18D2Onatural abundance10 %
Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 5.5, Details 13C / 15N-labeled HIV-1 CA DM


#NameIsotope labelingTypeConcentration
19HIV-1 CA[U-98% 13C; U-98% 15N]2 mM
20sodium chloridenatural abundance25 mM
21sodium acetate[U-99% 2H]25 mM
22DTT[U-99% 2H]10 mM
23sodium azidenatural abundance0.02 %
24AEBSF protease inhibitornatural abundance0.1 mM
25EDTA[U-99% 2H]1 mM
26D2Onatural abundance100 %
Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 5.5, Details 13C / 15N / 2H-labeled HIV-1 CA DM


#NameIsotope labelingTypeConcentration
27HIV-1 CA[U-98% 13C; U-98% 15N]2 mM
28sodium chloridenatural abundance25 mM
29sodium acetate[U-99% 2H]25 mM
30DTT[U-99% 2H]10 mM
31sodium azidenatural abundance0.02 %
32AEBSF protease inhibitornatural abundance0.1 mM
33EDTA[U-99% 2H]1 mM
34H2Onatural abundance90 %
35D2Onatural abundance10 %
Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 5.5, Details 13C / 15N / 2H-labeled HIV-1 CA DM


#NameIsotope labelingTypeConcentration
36HIV-1 CA[U-98% 13C; U-98% 15N]2 mM
37sodium chloridenatural abundance25 mM
38sodium acetate[U-99% 2H]25 mM
39DTT[U-99% 2H]10 mM
40sodium azidenatural abundance0.02 %
41AEBSF protease inhibitornatural abundance0.1 mM
42EDTA[U-99% 2H]1 mM
43D2Onatural abundance100 %
Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 5.5, Details 15N HIV-1 CA DM


#NameIsotope labelingTypeConcentration
44HIV-1 CA[U-98% 13C; U-98% 15N]2 mM
45sodium chloridenatural abundance25 mM
46sodium acetate[U-99% 2H]25 mM
47DTT[U-99% 2H]10 mM
48sodium azidenatural abundance0.02 %
49AEBSF protease inhibitornatural abundance0.1 mM
50EDTA[U-99% 2H]1 mM
51D2Onatural abundance100 %

LACS Plot; CA
Referencing offset: 0.54 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: 0.54 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: 0.07 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.04 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2LF4, Strand ID: A Detail


Release date
2011-10-24
Citation
Structure of a monomeric mutant of the HIV-1 capsid protein
Shin, R., Tzou, Y., Krishna, N.
Biochemistry (2011), 50, 9457-9467, PubMed 21995733 , DOI 10.1021/bi2011493 ,
Related entities 1. HIV-1 CA, : 1 : 76 : 115 entities Detail
Interaction partners 1. HIV-1 CA, : 4 interactors Detail
Keywords CA, capsid, fullerene, HIV-1