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BMRB: 17757

4KML

Backbone 1H and 13C Chemical Shift Assignments of human prion protein (residues 121-230) in its reduced state

Authors

Schwalbe, H., Schlepckow, K.

Assembly

human prion protein (residues 121-230)

Entity

1. human prion protein (residues 121-230) (polymer, Thiol state: all other bound), 113 monomers, 13354.78 Da Detail

GHMVVGGLGG YMLGSAMSRP IIHFGSDYED RYYRENMHRY PNQVYYRPMD EYSNQNNFVH DXVNITIKQH TVTTTTKGEN FTETDVKMME RVVEQMXITQ YERESQAYYQ RGS

Formula weight

13354.78 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 38.2 %, Completeness (bb): 78.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	38.2 % (454 of 1187)	28.6 % (195 of 683)	51.4 % (259 of 504)
Backbone	78.3 % (432 of 552)	78.1 % (178 of 228)	78.4 % (254 of 324)
Sidechain	14.0 % (103 of 737)	4.0 % (18 of 455)	30.1 % (85 of 282)
Aromatic	0.0 % (0 of 138)	0.0 % (0 of 69)	0.0 % (0 of 69)
Methyl	9.3 % (8 of 86)	9.3 % (4 of 43)	9.3 % (4 of 43)

1. hPrP(121-230)

GHMVVGGLGG YMLGSAMSRP IIHFGSDYED RYYRENMHRY PNQVYYRPMD EYSNQNNFVH DXVNITIKQH TVTTTTKGEN FTETDVKMME RVVEQMXITQ YERESQAYYQ RGS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 2.0, Details 8M urea pH 2

#	Name	Isotope labeling	Concentration
1	hPrP(121-230)	[U-13C; U-15N]	0.2 ~ 0.5 mM
2	TSP	natural abundance	1 mM
3	urea	natural abundance	8 M
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	TSP	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	TSP	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	TSP	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
¹H	TSP	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.26 ppm, Outliers: 3 Detail

Release date

2011-07-04

Citation

Unfolded-state structure and dynamics influence the fibril formation of human prion protein
Gerum, C., Silvers, R., Wirmer-Bartoschek, J., Schwalbe, H.
Angew. Chem Int Ed Engl. (2009), 48, 9452-9456, PubMed 19882604 , DOI 10.1002/anie.200903771 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17756 released on 2011-07-17
Title Backbone 1H and 13C Chemical Shift Assignments of human prion protein (residues 121-230) in its oxidized state

Related entities 1. human prion protein (residues 121-230), : 1 : 25 : 131 entities Detail

Interaction partners 1. human prion protein (residues 121-230), : 70 interactors Detail

More details for 70 interactors identified by 14 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC