Search

Query history

Search help 
Download

Found 1 Document (0.391 seconds)

BMRB: 17790


17790
2LG2
Structure of the duplex containing HNE derived (6S,8R,11S) N2-dG cyclic hemiacetal when placed opposite dT
Authors
Huang, H., Wang, H., Kozekova, A., LLoyd, R.S., Rizzo, C.J., Stone, M.P.
Assembly
(6S,8R,11S) gamma-hydroxy-1,N2-propano-deoxyguanosine adduct
Entity
1. DNA (5'-D(*GP*CP*TP*AP*GP*CP*GP*AP*GP*TP*CP*C)-3') (polymer, Thiol state: not present), 12 monomers, 3726.364 Da Detail

GCTAGCGAGT CC


2. DNA (5'-D(*GP*GP*AP*CP*TP*TP*GP*CP*TP*AP*GP*C)-3') (polymer, Thiol state: not present), 12 monomers, 3741.375 Da Detail

GGACTTGCTA GC


3. HNE (non-polymer), 156.222 Da
Total weight
7623.9614 Da
Max. entity weight
3741.375 Da
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 54.0 %, Completeness (bb): 52.4 % Detail
Download

Polymer type: polydeoxyribonucleotide

Total1H
All54.0 % (127 of 235)54.0 % (127 of 235)
Suger, PO452.4 % (88 of 168)52.4 % (88 of 168)
Nucleobase58.2 % (39 of 67)58.2 % (39 of 67)
Aromatic70.8 % (34 of 48)70.8 % (34 of 48)
Methyl100.0 % (5 of 5)100.0 % (5 of 5)

1. DNA (5'-D(*GP*CP*TP*AP*GP*CP*GP*AP*GP*TP*CP*C)-3')

GCTAGCGAGT CC

2. DNA (5'-D(*GP*GP*AP*CP*TP*TP*GP*CP*TP*AP*GP*C)-3')

GGACTTGCTA GC

Show sample condition
Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
1DNA_(5'-D(*GP*CP*TP*AP*GP*CP*GP*AP*GP*TP*CP*C)-3')natural abundance1 mM
2DNA_(5'-D(*GP*GP*AP*CP*TP*TP*GP*CP*TP*AP*GP*C)-3')natural abundance1 mM
3HNEnatural abundance1 mM
4D2Onatural abundance100 %

Release date
2012-08-29
Citation
Ring-chain tautomerization of trans-4-hydroxynonenal derived (6S,8R,11S) gamma-hydroxy-1,N2-propano-deoxyguanosine adduct when placed opposite 2'-deoxythymidine in duplex
Huang, H., Wang, H., Kozekova, A., LLoyd, R.S., Rizzo, C.J., Stone, M.P.
Not known
Experiments performed 1 experiments Detail
NMR combined restraints 3 contents Detail
Keywords HNE, HNE-dG, N2-dG cyclic hemiacetal, trans-4-hydroxynonenal