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Human Insulin Mutant A22Gly-B31Arg
Authors
Bocian, W., Kozerski, L.
Assembly
InsulinGR
Entity
1. InsulinGR 1 (polymer, Thiol state: all disulfide bound), 22 monomers, 2440.748 Da Detail

GIVEQCCTSI CSLYQLENYC NG


2. InsulinGR 2 (polymer, Thiol state: all disulfide bound), 31 monomers, 3586.105 Da Detail

FVNQHLCGSH LVEALYLVCG ERGFFYTPKT R


Total weight
6026.853 Da
Max. entity weight
3586.105 Da
Entity Connection
disulfide 3 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS6:SG1:CYS11:SG
2disulfidesing1:CYS7:SG2:CYS7:SG
3disulfidesing1:CYS20:SG2:CYS19:SG

Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 79.2 %, Completeness (bb): 74.5 % Detail

Polymer type: polypeptide(L)

Total1H13C
All79.2 % (439 of 554)93.9 % (295 of 314)60.0 % (144 of 240)
Backbone74.5 % (193 of 259)95.2 % (100 of 105)60.4 % (93 of 154)
Sidechain84.3 % (289 of 343)93.3 % (195 of 209)70.1 % (94 of 134)
Aromatic48.6 % (34 of 70)97.1 % (34 of 35) 0.0 % (0 of 35)
Methyl100.0 % (56 of 56)100.0 % (28 of 28)100.0 % (28 of 28)

1. InsulinGR 1

GIVEQCCTSI CSLYQLENYC NG

2. InsulinGR 2

FVNQHLCGSH LVEALYLVCG ERGFFYTPKT R

Sample #1

Solvent system 65% H2O / 35% CD3CN, Pressure 1 atm, Temperature 298 K, pH 3.5


#NameIsotope labelingTypeConcentration
1InsulinGR 1natural abundance2.0 ~ 3.0 mM
2InsulinGR 2natural abundance2.0 ~ 3.0 mM
3H2Onatural abundance65 %
4CD3CNnatural abundance35 %
Sample #2

Solvent system 65% D2O / 35% CD3CN, Pressure 1 atm, Temperature 298 K, pH 3.5


#NameIsotope labelingTypeConcentration
5InsulinGR 1natural abundance2.0 ~ 3.0 mM
6InsulinGR 2natural abundance2.0 ~ 3.0 mM
7D2Onatural abundance65 %
8CD3CNnatural abundance35 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2LGB, Strand ID: A, B Detail


Release date
2012-04-08
Citation
Recombinant A22(G)-B31 (R)-human insulin. A22 addition introduces conformational mobility in B chain C-terminus
Borowicz, P., Bednarek, E., Bocian, W., Sitkowski, J., Jaworska, B., Mikolajczyk, J., Glabski, T., Stadnik, D., Surmacz, W., Bogiel, M., Kozerski, L.
J. Biomol. NMR (2012), 52, 365-370, PubMed 22350952 , DOI 10.1007/s10858-012-9612-y ,
Related entities 1. InsulinGR 1, : 1 : 1 : 116 : 83 entities Detail
Related entities 2. InsulinGR 2, : 1 : 10 : 6 : 112 : 70 entities Detail
Interaction partners 1. InsulinGR 1, : 1 : 1 interactors Detail
Interaction partners 2. InsulinGR 2, : 6 : 1 interactors Detail
Experiments performed 4 experiments Detail
Keywords NMR structure, Recombinant A22G-B31R human insulin, water/acetonitrile solvent, Hormone, Human Insulin, mutant, water/acetonitrile solution