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BMRB: 17813

2LGL

NMR structure of the UHRF1 PHD domain

Authors

Wang, C.

Assembly

NMR structure of the UHRF1 PHD domain

Entity

1. NMR structure of the UHRF1 PHD domain, entity 1 (polymer, Thiol state: not reported), 69 monomers, 7839.816 Da Detail

SGPSCKHCKD DVNRLCRVCA CHLCGGRQDP DKQLMCDECD MAFHIYCLDP PLSSVPSEDE WYCPECRND

2. ZN (non-polymer), 65.409 × 3 Da

Total weight

8036.043 Da

Max. entity weight

7839.816 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 85.5 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.5 % (648 of 758)	84.2 % (336 of 399)	85.2 % (248 of 291)	94.1 % (64 of 68)
Backbone	95.3 % (383 of 402)	97.0 % (131 of 135)	92.6 % (189 of 204)	100.0 % (63 of 63)
Sidechain	78.0 % (329 of 422)	77.7 % (205 of 264)	80.4 % (123 of 153)	20.0 % (1 of 5)
Aromatic	58.0 % (29 of 50)	52.0 % (13 of 25)	62.5 % (15 of 24)	100.0 % (1 of 1)
Methyl	90.0 % (36 of 40)	90.0 % (18 of 20)	90.0 % (18 of 20)

1. entity 1

SGPSCKHCKD DVNRLCRVCA CHLCGGRQDP DKQLMCDECD MAFHIYCLDP PLSSVPSEDE WYCPECRND

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.5 mM
2	sodium chloride	natural abundance	137 mM
3	potassium phosphate	natural abundance	10 mM
4	DTT	natural abundance	3 mM
5	potassium chloride	natural abundance	2.7 mM
6	sodium azide	natural abundance	0.1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.61 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.61 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LGL, Strand ID: A Detail

Release date

2011-08-31

Citation

Structural basis for site-specific reading of unmodified R2 of histone H3 tail by UHRF1 PHD finger
Wang, C., Shen, J., Yang, Z., Chen, P., Zhao, B., Hu, W., Lan, W., Tong, X., Wu, H., Li, G., Cao, C.
Cell Res. (2011), 21, 1379-1382, PubMed 21808299 , DOI 10.1038/cr.2011.123 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 17808 released on 2011-12-11
    Title Structure of PHD domain of UHRF1 in complex with H3 peptide
  BMRB: 17812 released on 2011-08-31
    Title NMR STRUCTURE OF UHRF1 PHD DOMAINS IN A COMPLEX WITH HISTONE H3 PEPTIDE
  Swiss-Prot: Q96T88 released on 2005-06-07
    Title UHRF1_HUMAN Entity E3 ubiquitin-protein ligase UHRF1

Related entities 1. NMR structure of the UHRF1 PHD domain, entity 1, : 1 : 12 : 2 : 46 entities Detail

Interaction partners 1. NMR structure of the UHRF1 PHD domain, entity 1, : 25 interactors Detail

More details for 25 interactors identified by 8 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC