BMRB: 17843

GB98-T25I solution structure

Authors

He, Y., Chen, Y., Alexander, P., Bryan, P., Orban, J.

Assembly

GB98-T25I

Entity

1. GB98-T25I (polymer, Thiol state: not present), 56 monomers, 6408.341 Da Detail

TTYKLILNLK QAKEEAIKEL VDAGIAEKYF KLIANAKTVE GVWTYKDEIK TFTVTE

Formula weight

6408.341 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 35.7 %, Completeness (bb): 71.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	35.7 % (245 of 687)	26.6 % (94 of 354)	37.7 % (103 of 273)	80.0 % (48 of 60)
Backbone	71.4 % (240 of 336)	79.8 % (91 of 114)	61.4 % (102 of 166)	83.9 % (47 of 56)
Sidechain	5.9 % (24 of 405)	1.3 % (3 of 240)	12.4 % (20 of 161)	25.0 % (1 of 4)
Aromatic	3.6 % (2 of 56)	3.6 % (1 of 28)	0.0 % (0 of 27)	100.0 % (1 of 1)
Methyl	1.2 % (1 of 82)	0.0 % (0 of 41)	2.4 % (1 of 41)

1. entity

TTYKLILNLK QAKEEAIKEL VDAGIAEKYF KLIANAKTVE GVWTYKDEIK TFTVTE

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 278 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	GB98-T25I	[U-100% 13C; U-100% 15N]		0.1 ~ 0.3 mM
2	potassium phosphate	natural abundance		100 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 2LHG, Strand ID: A Detail

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Release date

2012-02-27

Citation

Mutational tipping points for switching protein folds and functions
He, Y., Chen, Y., Alexander, P., Bryan, P., Orban, J.
Structure (2012), 20, 283-291, PubMed 22325777 , DOI 10.1016/j.str.2011.11.018 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. GB98-T25I, : 1 : 7 : 101 entities Detail

Experiments performed 5 experiments Detail