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Backbone 1H, 13C, and 15N chemical shift assignments of oxidized yeast iso-1 cytochrome c
Authors
Volkov, A.N., Van de Water, K., Vanwetswinkel, S., van Nuland, N.A. J.
Assembly
yCc
Entity
1. yCc (polymer, Thiol state: all other bound), 108 monomers, 12018.58 Da Detail

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE


2. HEC (non-polymer), 618.503 Da
Total weight
12637.083 Da
Max. entity weight
12018.58 Da
Entity Connection
na 2 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS19:SG2:HEC1:HEC
2nasing1:CYS22:SG2:HEC1:HEC

Source organism
Saccharomyces cerevisiae
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 100.0 %, Completeness: 43.8 %, Completeness (bb): 79.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All43.8 % (559 of 1277)21.0 % (141 of 670)62.7 % (307 of 490)94.9 % (111 of 117)
Backbone79.5 % (499 of 628)45.3 % (96 of 212)96.5 % (301 of 312)98.1 % (102 of 104)
Sidechain20.8 % (155 of 745) 9.8 % (45 of 458)36.9 % (101 of 274)69.2 % (9 of 13)
Aromatic 0.0 % (0 of 108) 0.0 % (0 of 54) 0.0 % (0 of 53) 0.0 % (0 of 1)
Methyl10.9 % (10 of 92) 4.3 % (2 of 46)17.4 % (8 of 46)

1. yCc

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0


#NameIsotope labelingTypeConcentration
1yCc[U-100% 13C; U-100% 15N]1 mM
2sodium phosphatenatural abundance20 mM
3sodium chloridenatural abundance100 mM
4H2Onatural abundance95 %
5D2Onatural abundance5 %
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0


#NameIsotope labelingTypeConcentration
6yCc[U-100% 13C; U-100% 15N]1 mM
7sodium phosphatenatural abundance20 mM
8H2Onatural abundance95 %
9D2Onatural abundance5 %

Chem. Shift Complete2
Sequence coverage: 93.5 %, Completeness: 34.0 %, Completeness (bb): 58.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All34.0 % (869 of 2554)22.2 % (298 of 1340)35.7 % (350 of 980)94.4 % (221 of 234)
Backbone58.2 % (731 of 1256)46.2 % (196 of 424)53.2 % (332 of 624)97.6 % (203 of 208)
Sidechain16.1 % (240 of 1490)11.1 % (102 of 916)21.9 % (120 of 548)69.2 % (18 of 26)
Aromatic 4.6 % (10 of 216) 4.6 % (5 of 108) 4.7 % (5 of 106) 0.0 % (0 of 2)
Methyl 8.2 % (15 of 184) 4.3 % (4 of 92)12.0 % (11 of 92)

1. yCc

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0


#NameIsotope labelingTypeConcentration
1yCc[U-100% 13C; U-100% 15N]1 mM
2sodium phosphatenatural abundance20 mM
3sodium chloridenatural abundance100 mM
4H2Onatural abundance95 %
5D2Onatural abundance5 %
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0


#NameIsotope labelingTypeConcentration
6yCc[U-100% 13C; U-100% 15N]1 mM
7sodium phosphatenatural abundance20 mM
8H2Onatural abundance95 %
9D2Onatural abundance5 %

Release date
2012-03-13
Citation
Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopy
Volkov, A.N., Vanwetswinkel, S., Van de Water, K., van Nuland, N.A. J.
J. Biomol. NMR (2012), 52, 245-256, PubMed 22318343 , DOI 10.1007/s10858-012-9607-8 ,
Entries sharing articles BMRB: 3 entries Detail
  BMRB: 17846 released on 2012-03-13
    Title Backbone 1H, 13C, and 15N chemical shift assignments of reduced yeast iso-1 cytochrome c
  BMRB: 17847 released on 2012-03-13
    Title Backbone 1H, 13C, and 15N chemical shift assignments of oxidized horse heart cytochrome c
  BMRB: 17848 released on 2012-03-13
    Title Backbone 1H, 13C, and 15N chemical shift assignments of reduced horse heart cytochrome c
Related entities 1. yCc, : 1 : 11 : 42 : 146 entities Detail
Interaction partners 1. yCc, : 4 interactors Detail
Experiments performed 6 experiments Detail