Backbone 1H, 13C, and 15N chemical shift assignments of oxidized yeast iso-1 cytochrome c
AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS19:SG | 2:HEC1:HEC |
2 | na | sing | 1:CYS22:SG | 2:HEC1:HEC |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 44.1 % (562 of 1274) | 21.2 % (142 of 670) | 63.1 % (309 of 490) | 97.4 % (111 of 114) |
Backbone | 79.8 % (511 of 640) | 47.8 % (107 of 224) | 96.8 % (302 of 312) | 98.1 % (102 of 104) |
Sidechain | 20.0 % (146 of 730) | 7.8 % (35 of 446) | 37.2 % (102 of 274) | 90.0 % (9 of 10) |
Aromatic | 0.9 % (1 of 108) | 1.9 % (1 of 54) | 0.0 % (0 of 53) | 0.0 % (0 of 1) |
Methyl | 9.8 % (9 of 92) | 2.2 % (1 of 46) | 17.4 % (8 of 46) |
1. yCc
AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATESolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | yCc | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | H2O | natural abundance | 95 % | |
5 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | yCc | [U-100% 13C; U-100% 15N] | 1 mM | |
7 | sodium phosphate | natural abundance | 20 mM | |
8 | H2O | natural abundance | 95 % | |
9 | D2O | natural abundance | 5 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 33.6 % (857 of 2548) | 22.0 % (295 of 1340) | 34.9 % (342 of 980) | 96.5 % (220 of 228) |
Backbone | 57.8 % (740 of 1280) | 48.0 % (215 of 448) | 51.8 % (323 of 624) | 97.1 % (202 of 208) |
Sidechain | 14.9 % (217 of 1460) | 8.9 % (79 of 892) | 21.9 % (120 of 548) | 90.0 % (18 of 20) |
Aromatic | 4.2 % (9 of 216) | 4.6 % (5 of 108) | 3.8 % (4 of 106) | 0.0 % (0 of 2) |
Methyl | 9.2 % (17 of 184) | 5.4 % (5 of 92) | 13.0 % (12 of 92) |
1. yCc
AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATESolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | yCc | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | H2O | natural abundance | 95 % | |
5 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | yCc | [U-100% 13C; U-100% 15N] | 1 mM | |
7 | sodium phosphate | natural abundance | 20 mM | |
8 | H2O | natural abundance | 95 % | |
9 | D2O | natural abundance | 5 % |