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BMRB: 17846

6RSJ

Backbone 1H, 13C, and 15N chemical shift assignments of reduced yeast iso-1 cytochrome c

Authors

Volkov, A.N., Van de Water, K., Vanwetswinkel, S., van Nuland, N.A. J.

Assembly

yCc

Entity

1. yCc (polymer, Thiol state: all other bound), 108 monomers, 12018.58 Da Detail

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

2. HEC (non-polymer), 618.503 Da

Total weight

12637.083 Da

Max. entity weight

12018.58 Da

Entity Connection

na 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS19:SG	2:HEC1:HEC
2	na	sing	1:CYS22:SG	2:HEC1:HEC

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 47.3 %, Completeness (bb): 80.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.3 % (603 of 1274)	26.6 % (178 of 670)	64.1 % (314 of 490)	97.4 % (111 of 114)
Backbone	80.2 % (513 of 640)	50.0 % (112 of 224)	95.8 % (299 of 312)	98.1 % (102 of 104)
Sidechain	25.3 % (185 of 730)	14.8 % (66 of 446)	40.1 % (110 of 274)	90.0 % (9 of 10)
Aromatic	0.0 % (0 of 108)	0.0 % (0 of 54)	0.0 % (0 of 53)	0.0 % (0 of 1)
Methyl	15.2 % (14 of 92)	8.7 % (4 of 46)	21.7 % (10 of 46)

1. yCc

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	yCc	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	yCc	[U-100% 13C; U-100% 15N]	1 mM
7	sodium phosphate	natural abundance	20 mM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

Chem. Shift Complete2

Sequence coverage: 94.4 %, Completeness: 36.7 %, Completeness (bb): 59.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	36.7 % (936 of 2548)	26.9 % (361 of 1340)	36.0 % (353 of 980)	97.4 % (222 of 228)
Backbone	59.7 % (764 of 1280)	51.6 % (231 of 448)	52.7 % (329 of 624)	98.1 % (204 of 208)
Sidechain	19.0 % (277 of 1460)	14.6 % (130 of 892)	23.5 % (129 of 548)	90.0 % (18 of 20)
Aromatic	0.0 % (0 of 216)	0.0 % (0 of 108)	0.0 % (0 of 106)	0.0 % (0 of 2)
Methyl	16.8 % (31 of 184)	16.3 % (15 of 92)	17.4 % (16 of 92)

1. yCc

AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	yCc	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	yCc	[U-100% 13C; U-100% 15N]	1 mM
7	sodium phosphate	natural abundance	20 mM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

Release date

2012-03-13

Citation

Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopy
Volkov, A.N., Vanwetswinkel, S., Van de Water, K., van Nuland, N.A. J.
J. Biomol. NMR (2012), 52, 245-256, PubMed 22318343 , DOI 10.1007/s10858-012-9607-8 , Abstract

Related entities 1. yCc, : 1 : 22 : 45 : 169 entities Detail

Interaction partners 1. yCc, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC