BMRBj - BMREntryMaster

Found 1 Document (4.518 seconds)

BMRB: 17878

Backbone 1H, 13C, and 15N Chemical Shift Assignments for La(4-325)

Authors

Martino, L., Kelly, G., Curry, S., Conte, M.R.

Assembly

La(4-325)

Entity

1. La(4-325) (polymer, Thiol state: all free), 322 monomers, 37441.28 Da Detail

NGDNEKMAAL EAKICHQIEY YFGDFNLPRD KFLKEQIKLD EGWVPLEIMI KFNRLNRLTT DFNVIVEALS KSKAELMEIS EDKTKIRRSP SKPLPEVTDE YKNDVKNRSV YIKGFPTDAT LDDIKEWLED KGQVLNIQMR RTLHKAFKGS IFVVFDSIES AKKFVETPGQ KYKETDLLIL FKDDYFAKKN EERKQNKVEA KLRAKQEQEA KQKLEEDAEM KSLEEKIGCL LKFSGDLDDQ TCREDLHILF SNHGEIKWID FVRGAKEGII LFKEKAKEAL GKAKDANNGN LQLRNKEVTW EVLEGEVEKE ALKKIIEDQQ ES

Formula weight

37441.28 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.1 %, Completeness: 39.3 %, Completeness (bb): 68.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	39.3 % (1558 of 3968)	23.2 % (487 of 2099)	52.5 % (798 of 1521)	78.4 % (273 of 348)
Backbone	68.5 % (1314 of 1918)	48.8 % (318 of 652)	76.4 % (727 of 951)	85.4 % (269 of 315)
Sidechain	20.2 % (475 of 2357)	11.7 % (170 of 1447)	34.3 % (301 of 877)	12.1 % (4 of 33)
Aromatic	5.1 % (14 of 272)	6.6 % (9 of 136)	3.8 % (5 of 132)	0.0 % (0 of 4)
Methyl	23.2 % (77 of 332)	19.3 % (32 of 166)	27.1 % (45 of 166)

1. human La protein

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.25, Details 20 mM Tris, 100 mM KCl, 1 mM DTT pH 7.25

#	Name	Isotope labeling	Concentration
1	La(4-325)	[U-100% 13C; U-100% 15N; U-80% 2H]	0.4 mM
2	Tris	natural abundance	20 mM
3	KCl	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.45 ppm, Outliers: 1 Detail

Release date

2011-12-08

Citation

Analysis of the interaction with the hepatitis C virus mRNA reveals an alternative mode of RNA recognition by the human La protein
Martino, L., Pennell, S., Kelly, G., Bui, T.T.T., Kotik-Kogan, O., Smerdon, S.J., Drake, A.F., Curry, S., Conte, M.R.
Nucleic Acids Res. (2012), 40, 1381-1394, PubMed 22009680 , DOI 10.1093/nar/gkr890 , Abstract

Related entities 1. La(4-325), : 2 : 17 entities Detail

Interaction partners 1. La(4-325), : 87 interactors Detail

More details for 87 interactors identified by 30 citations

Experiments performed 5 experiments Detail

1 2D 1H-15N TROSY-HSQC