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Dynamics of isolated C domain of calmodulin apo form
Authors
Wang, X., Kleerekoper, Q.K., Putkey, J.A.
Assembly
C-CaM apo form
Entity
1. C-CaM apo form (polymer, Thiol state: not present), 71 monomers, 8147.759 Da Detail

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K


Formula weight
8147.759 Da
Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 28.8 %, Completeness (bb): 64.3 % Detail

Polymer type: polypeptide(L)

Total1H15N
All28.8 % (142 of 493)17.2 % (71 of 413)88.7 % (71 of 80)
Backbone64.3 % (137 of 213)46.5 % (66 of 142)100.0 % (71 of 71)
Sidechain 1.8 % (5 of 280) 1.8 % (5 of 271) 0.0 % (0 of 9)
Aromatic 0.0 % (0 of 25) 0.0 % (0 of 25)
Methyl 0.0 % (0 of 33) 0.0 % (0 of 33)

1. isolcated c domain of calmodulin

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details apo 15N labeled isolated C-domain of calmodulin(Residues 78-148).


#NameIsotope labelingTypeConcentration
1isolcated c domain of calmodulin[U-100% 15N]0.5 mM
2EDTAnatural abundance10 mM
3potassium chloridenatural abundance100 mM
4H2Onatural abundance95 %
5D2Onatural abundance5 %

Heteronucl. T1
69 T1 values in 1 lists
Coherence Sz, Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Heteronucl. T2
69 T2 values in 1 lists
Coherence S(+,-), Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Heteronucl. NOE
69 NOE values in 1 lists
Value type relative intensities, Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Heteronucl. T1/T2
69 T1/T2 values in 1 lists
Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Release date
2011-10-09
Citation
Intrinsically disordered PEP-19 confers unique dynamic properties to apo and calcium calmodulin
Wang, X., Kleerekoper, Q.K., Xiong, L., Putkey, J.A.
Biochemistry (2010), 49, 10287-10297, PubMed 20973509 , DOI 10.1021/bi100500m ,
Entries sharing articles BMRB: 3 entries Detail
  BMRB: 17981 released on 2011-10-09
    Title Dynamics of isolated C domain of calmodulin complexed with PEP-19 in the absence of Ca2+
  BMRB: 17982 released on 2011-10-09
    Title Dynamics of isolated C domain of calmodulin complexed with Ca2+
  BMRB: 17983 released on 2011-10-09
    Title Dynamics of isolated C domain of calmodulin complexed with PEP-19 in the presence of Ca2+
Related entities 1. C-CaM apo form, : 2 : 102 : 4 : 3 : 279 entities Detail
Interaction partners 1. C-CaM apo form, : 1 interactors Detail
Experiments performed 11 experiments Detail
Keywords assignment, backbone dynamics, calcium binding, calmodulin, PEP-19