Search

NMR Solution Structure of Yeast Iso-1-cytochrome c Mutant P71H in reduced states
Authors
Lan, W., Wang, Z., Yang, Z., Zhu, J., Ying, T., Jiang, X., Zhang, X., Wu, H., Liu, M., Tan, X., Cao, C., Huang, Z.
Assembly
Iso-1-cytochrome
Entity
1. Iso-1-cytochrome (polymer, Thiol state: all free), 108 monomers, 12031.53 Da Detail

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNHAKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE


2. HEC (non-polymer, Thiol state: not present), 618.503 Da
Total weight
12650.033 Da
Max. entity weight
12031.53 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.2 %, Completeness: 78.0 %, Completeness (bb): 93.9 % Detail

Polymer type: polypeptide(L)

Total1H
All78.0 % (517 of 663)78.0 % (517 of 663)
Backbone93.9 % (200 of 213)93.9 % (200 of 213)
Sidechain70.4 % (317 of 450)70.4 % (317 of 450)
Aromatic75.0 % (42 of 56)75.0 % (42 of 56)
Methyl95.7 % (45 of 47)95.7 % (45 of 47)

1. Iso-1-cytochrome

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNHAKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0


#NameIsotope labelingTypeConcentration
1Iso-1-cytochromenatural abundance1 mM
2H2Onatural abundance90 %
3D2Onatural abundance10 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0


#NameIsotope labelingTypeConcentration
4Iso-1-cytochromenatural abundance1 mM
5D2Onatural abundance100 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2LIT, Strand ID: A Detail


Release date
2011-12-05
Citation
Conformational toggling of yeast iso-1-cytochrome C in the oxidized and reduced states
Lan, W., Wang, Z., Yang, Z., Zhu, J., Ying, T., Jiang, X., Zhang, X., Wu, H., Liu, M., Tan, X., Cao, C., Huang, Z.
PLoS One (2011), 6, e27219-e27219, PubMed 22087268 , DOI 10.1371/journal.pone.0027219 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 17903 released on 2011-12-05
    Title NMR Solution Structure of Yeast Iso-1-cytochrome c Mutant P71H in oxidized states
Related entities 1. Iso-1-cytochrome, : 1 : 1 : 53 : 145 entities Detail
Interaction partners 1. Iso-1-cytochrome, : 4 interactors Detail
Keywords cytochrome c, NMR, P71H, reduced