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1H, 13C and 15N backbone resonance assignment of the activated p38 mitogen-activated protein kinase
Authors
Nielsen, G., Schwalbe, H.
Assembly
p38alpha
Entity
1. p38alpha (polymer, Thiol state: all free), 349 monomers, 40016.31 Da Detail

NSQERPTFYR QELNKTIWEV PERYQNLSPV GSGAYGSVCA AFDTKTGHRV AVKKLSRPFQ SIIHAKRTYR ELRLLKHMKH ENVIGLLDVF TPARSLEEFN DVYLVTHLMG ADLNNIVKCQ KLTDDHVQFL IYQILRGLKY IHSADIIHRD LKPSNLAVNE DCELKILDFG LARHTDDEMT GYVATRWYRA PEIMLNWMHY NQTVDIWSVG CIMAELLTGR TLFPGTDHID QLKLILRLVG TPGAELLKKI SSESARNYIQ SLAQMPKMNF ANVFIGANPL AVDLLEKMLV LDSDKRITAA QALAHAYFAQ YHDPDDEPVA DPYDQSFESR DLLIDEWKSL TYDEVISFV


Formula weight
40016.31 Da
Source organism
Mus musculus
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 72.2 %, Completeness: 34.5 %, Completeness (bb): 55.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All34.5 % (1440 of 4173)23.0 % (494 of 2151)44.6 % (729 of 1634)55.9 % (217 of 388)
Backbone55.7 % (1143 of 2051)39.8 % (272 of 684)63.5 % (655 of 1032)64.5 % (216 of 335)
Sidechain20.8 % (511 of 2456)15.1 % (221 of 1467)30.8 % (288 of 936) 3.8 % (2 of 53)
Aromatic 3.9 % (14 of 362) 5.5 % (10 of 181) 2.3 % (4 of 176) 0.0 % (0 of 5)
Methyl22.2 % (95 of 428)19.6 % (42 of 214)24.8 % (53 of 214)

1. p38alpha

NSQERPTFYR QELNKTIWEV PERYQNLSPV GSGAYGSVCA AFDTKTGHRV AVKKLSRPFQ SIIHAKRTYR ELRLLKHMKH ENVIGLLDVF TPARSLEEFN DVYLVTHLMG ADLNNIVKCQ KLTDDHVQFL IYQILRGLKY IHSADIIHRD LKPSNLAVNE DCELKILDFG LARHTDDEMT GYVATRWYRA PEIMLNWMHY NQTVDIWSVG CIMAELLTGR TLFPGTDHID QLKLILRLVG TPGAELLKKI SSESARNYIQ SLAQMPKMNF ANVFIGANPL AVDLLEKMLV LDSDKRITAA QALAHAYFAQ YHDPDDEPVA DPYDQSFESR DLLIDEWKSL TYDEVISFV

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details activated by double phosphorylation (pT180 and pY182)


#NameIsotope labelingTypeConcentration
1p38alpha[U-13C; U-15N; U-2H]0.5 mM

Release date
2011-10-23
Citation
NMR spectroscopic investigations of the activated p38α mitogen-activated protein kinase
Nielsen, G., Schwalbe, H.
Chembiochem. (2011), 12, 2599-2607, PubMed 22012687 , DOI 10.1002/cbic.201100527 ,
Related entities 1. p38alpha, : 1 : 7 : 91 : 102 entities Detail
Interaction partners 1. p38alpha, : 28 interactors Detail
Experiments performed 4 experiments Detail
Keywords activation, assignment, NMR, p38, phosphorylation