BMRB: 17981

Dynamics of isolated C domain of calmodulin complexed with PEP-19 in the absence of Ca2+

Authors

Wang, X., Kleerekoper, Q.K., Putkey, J.A.

Assembly

C-CaM/PEP-19 complex

Entity

1. isolcated c domain of calmodulin (polymer, Thiol state: not present), 71 monomers, 8147.759 Da Detail

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K

2. PEP-19 (polymer, Thiol state: not present), 62 monomers, 6775.333 Da Detail

MAERQGAGAT NGKDKTSGEN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS

Total weight

14923.092 Da

Max. entity weight

8147.759 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 51.9 %, Completeness: 14.8 %, Completeness (bb): 33.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	14.8 % (138 of 932)	8.8 % (69 of 786)	47.3 % (69 of 146)
Backbone	33.8 % (138 of 408)	25.0 % (69 of 276)	52.3 % (69 of 132)
Sidechain	0.0 % (0 of 524)	0.0 % (0 of 510)	0.0 % (0 of 14)
Aromatic	0.0 % (0 of 40)	0.0 % (0 of 40)
Methyl	0.0 % (0 of 52)	0.0 % (0 of 52)

1. isolcated c domain of calmodulin

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K

2. PEP-19

MAERQGAGAT NGKDKTSGEN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details 15N labeled apo isolated C-domain of CaM in complex with PEP-19

#	Name	Isotope labeling	Type	Concentration
1	isolcated c domain of calmodulin	[U-100% 15N]		0.5 mM
2	PEP-19	natural abundance		0.8 mM
3	EDTA	natural abundance		10 mM
4	potassium chloride	natural abundance		100 mM
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

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Heteronucl. T₁

62 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Experiment name T1

Heteronucl. T₂

63 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Experiment name T2

Heteronucl. NOE

63 NOE values in 1 lists
Value type relative intensities, Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Experiment name NOE

Heteronucl. T₁/T₂

62 T₁/T₂ values in 1 lists
Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Release date

2011-10-09

Citation

Intrinsically disordered PEP-19 confers unique dynamic properties to apo and calcium calmodulin
Wang, X., Kleerekoper, Q.K., Xiong, L., Putkey, J.A.
Biochemistry (2010), 49, 10287-10297, PubMed 20973509 , DOI 10.1021/bi100500m , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. isolcated c domain of calmodulin, : 11 : 174 entities Detail

Related entities 2. PEP-19, : 2 : 12 entities Detail

Interaction partners 2. PEP-19, : 5 interactors Detail

Experiments performed 11 experiments Detail

Chemical shift validation 4 contents Detail