BMRB: 17982

Dynamics of isolated C domain of calmodulin complexed with Ca2+

Authors

Wang, X., Kleerekoper, Q.K., Putkey, J.A.

Assembly

C-CaM/Ca complex

Entity

1. isolcated c domain of calmodulin (polymer, Thiol state: not present), 71 monomers, 8147.759 Da Detail

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K

2. CA (non-polymer), 40.078 Da

Total weight

8187.837 Da

Max. entity weight

8147.759 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 35.4 %, Completeness (bb): 67.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	35.4 % (173 of 489)	24.7 % (102 of 413)	93.4 % (71 of 76)
Backbone	67.9 % (148 of 218)	52.4 % (77 of 147)	100.0 % (71 of 71)
Sidechain	9.2 % (25 of 271)	9.4 % (25 of 266)	0.0 % (0 of 5)
Aromatic	16.0 % (4 of 25)	16.0 % (4 of 25)
Methyl	6.1 % (2 of 33)	6.1 % (2 of 33)

1. isolcated c domain of calmodulin

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details 15N labeled Ca2+ bound isolated C-domain of calmodulin(Residues 78-148)

#	Name	Isotope labeling	Type	Concentration
1	isolcated c domain of calmodulin	[U-100% 15N]		0.5 mM
2	calcium	natural abundance		5 mM
3	potassium chloride	natural abundance		100 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

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Heteronucl. T₁

66 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Experiment name T1

Heteronucl. T₂

65 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Experiment name T2

Heteronucl. NOE

68 NOE values in 1 lists
Value type relative intensities, Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Experiment name NOE

Heteronucl. T₁/T₂

65 T₁/T₂ values in 1 lists
Field strength (¹H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail

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Pressure 1 atm, Temperature 298 K, pH 6.3

Release date

2011-10-09

Citation

Intrinsically disordered PEP-19 confers unique dynamic properties to apo and calcium calmodulin
Wang, X., Kleerekoper, Q.K., Xiong, L., Putkey, J.A.
Biochemistry (2010), 49, 10287-10297, PubMed 20973509 , DOI 10.1021/bi100500m , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. isolcated c domain of calmodulin, : 2 : 113 : 4 : 3 : 453 entities Detail

Interaction partners 1. isolcated c domain of calmodulin, : 1 interactors Detail

Experiments performed 11 experiments Detail

Chemical shift validation 4 contents Detail