Search

Dynamics of isolated C domain of calmodulin complexed with PEP-19 in the presence of Ca2+
Authors
Wang, X., Kleerekoper, Q.K., Putkey, J.A.
Assembly
C-CaM/PEP-19 complex
Entity
1. isolcated c domain of calmodulin (polymer, Thiol state: not present), 71 monomers, 8147.759 Da Detail

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K


2. PEP-19 (polymer, Thiol state: not present), 62 monomers, 6775.333 Da Detail

MAERQGAGAT NGKDKTSGEN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS


3. CA (non-polymer), 40.078 Da
Total weight
14963.17 Da
Max. entity weight
8147.759 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation
Chem. Shift Complete
Sequence coverage: 51.9 %, Completeness: 17.0 %, Completeness (bb): 34.8 % Detail

Polymer type: polypeptide(L)

Total1H15N
All17.0 % (160 of 939)11.8 % (93 of 786)43.8 % (67 of 153)
Backbone34.8 % (138 of 397)26.8 % (71 of 265)50.8 % (67 of 132)
Sidechain 4.2 % (23 of 542) 4.4 % (23 of 521) 0.0 % (0 of 21)
Aromatic 0.0 % (0 of 40) 0.0 % (0 of 40)
Methyl 9.6 % (5 of 52) 9.6 % (5 of 52)

1. isolcated c domain of calmodulin

DTDSEEEIRE AFRVFDKDGN GYISAAELRH VMTNLGEKLT DEEVDEMIRE ADIDGDGQVN YEEFVQMMTA K

2. PEP-19

MAERQGAGAT NGKDKTSGEN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details 15N labeled isolated C-domain of CaM in complex with PEP-19


#NameIsotope labelingTypeConcentration
1isolcated c domain of calmodulinnatural abundance0.5 mM
2PEP-19natural abundance0.8 mM
3calciumnatural abundance5 mM
4potassium chloridenatural abundance100 mM
5H2Onatural abundance95 %
6D2Onatural abundance5 %

Heteronucl. T1
63 T1 values in 1 lists
Coherence Sz, Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Heteronucl. T2
64 T2 values in 1 lists
Coherence S(+,-), Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Heteronucl. NOE
63 NOE values in 1 lists
Value type relative intensities, Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Heteronucl. T1/T2
62 T1/T2 values in 1 lists
Field strength (1H) 600.13 MHz, Pressure 1 atm, Temperature 298 K, pH 6.3 Detail
Release date
2011-10-09
Citation
Intrinsically disordered PEP-19 confers unique dynamic properties to apo and calcium calmodulin
Wang, X., Kleerekoper, Q.K., Xiong, L., Putkey, J.A.
Biochemistry (2010), 49, 10287-10297, PubMed 20973509 , DOI 10.1021/bi100500m ,
Entries sharing articles BMRB: 3 entries Detail
  BMRB: 17881 released on 2011-10-09
    Title Dynamics of isolated C domain of calmodulin apo form
  BMRB: 17981 released on 2011-10-09
    Title Dynamics of isolated C domain of calmodulin complexed with PEP-19 in the absence of Ca2+
  BMRB: 17982 released on 2011-10-09
    Title Dynamics of isolated C domain of calmodulin complexed with Ca2+
Related entities 2. PEP-19, : 1 : 6 entities Detail
Interaction partners 2. PEP-19, : 5 interactors Detail
Keywords assignment, backbone dynamics, calcium binding, calmodulin, PEP-19