BMRB: 18007

TBA

Authors

Tripsianes, K., Madl, T., Machyna, M., Fessas, D., Englbrecht, C., Neugebauer, U., Sattler, M.

Assembly

Molecular system

Entity

1. A (polymer, Thiol state: all free), 64 monomers, 7088.850 Da Detail

NTAASLQQWK VGDKCSAIWS EDGCIYPATI ASIDFKRETC VVVYTGYGNR EEQNLSDLLS PICE

2. DA2 (non-polymer), 202.254 Da

Total weight

7291.104 Da

Max. entity weight

7088.85 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.2 %, Completeness (bb): 84.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.2 % (655 of 718)	98.4 % (363 of 369)	80.3 % (224 of 279)	97.1 % (68 of 70)
Backbone	84.7 % (322 of 380)	98.5 % (128 of 130)	71.3 % (134 of 188)	96.8 % (60 of 62)
Sidechain	98.7 % (393 of 398)	98.3 % (235 of 239)	99.3 % (150 of 151)	100.0 % (8 of 8)
Aromatic	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (27 of 27)	100.0 % (2 of 2)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. A

NTAASLQQWK VGDKCSAIWS EDGCIYPATI ASIDFKRETC VVVYTGYGNR EEQNLSDLLS PICE

Show sample condition

Sample

Solvent system 93% WATER/7% D2O, Pressure 1.000 atm, Temperature 298 K, pH 6.5, Details 4mM SMN 18mM Da2

#	Name	Isotope labeling	Type	Concentration
1	SMN	[U-13C; U-15N]		4 mM
2	DA2	natural abundance		18 mM
3	H2O	natural abundance		93 %
4	D2O	natural abundance		7 %
5	phosphate	natural abundance		20 mM
6	NaCl	natural abundance		50 mM

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LACS Plot; CA

Referencing offset: -0.38 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.38 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 4A4G, Strand ID: A Detail

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Release date

2011-11-01

Citation

Structural basis for dimethylarginine recognition by the Tudor domains of human SMN and SPF30 proteins
Tripsianes, K., Madl, T., Machyna, M., Fessas, D., Englbrecht, C., Fischer, U., Neugebauer, K., Sattler, M.
Nat. Struct. Mol. Biol. (2011), 18, 1414-1420, PubMed 22101937 , DOI 10.1038/nsmb.2185 , Abstract

Related entities 1. A, : 1 : 8 : 1 : 56 entities Detail

Interaction partners 1. A, : 161 interactors Detail

Experiments performed 6 experiments Detail