SOLUTION NMR-DERIVED STRUCTURE OF CALMODULIN C-LOBE BOUND WITH ER ALPHA PEPTIDE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 44.5 % (446 of 1003) | 46.3 % (244 of 527) | 35.2 % (135 of 384) | 72.8 % (67 of 92) |
Backbone | 65.6 % (336 of 512) | 78.3 % (137 of 175) | 52.2 % (132 of 253) | 79.8 % (67 of 84) |
Sidechain | 30.1 % (172 of 572) | 30.4 % (107 of 352) | 30.7 % (65 of 212) | 0.0 % (0 of 8) |
Aromatic | 6.5 % (4 of 62) | 6.5 % (2 of 31) | 6.7 % (2 of 30) | 0.0 % (0 of 1) |
Methyl | 12.5 % (10 of 80) | 12.5 % (5 of 40) | 12.5 % (5 of 40) |
1. Calmodulin C-Lobe
EEEIREAFRV FDKDGNGYIS AAELRHVMTN LGEKLTDEEV DEMIREADID GDGQVNYEEF VQMMTAK2. ER alpha peptide
RAANLWPSPL MIKRSKKNSSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cal_C | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | H2O | natural abundance | 93 % | |
3 | D2O | natural abundance | 7 % | |
4 | Tris-d11 | natural abundance | 20 mM | |
5 | sodium chloride | natural abundance | 50 mM | |
6 | CaCl2 | natural abundance | 5 mM |