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BMRB: 18092

2LLX

Solution structure of the N-terminal domain of human polypeptide chain release factor eRF1

Authors

Polshakov, V.I., Eliseev, B.D., Birdsall, B., Frolova, L.YU.

Assembly

NeRF1

Entity

1. NeRF1 (polymer, Thiol state: all free), 150 monomers, 16742.11 Da Detail

MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK MLADEFGTAS NIKSRVNRLS VLGAITSVQQ RLKLYNKVPP NGLVVYCGTI VTEEGKEKKV NIDFEPFKPI NTSLYLCDNK FHTEALTALL SDLEHHHHHH

Formula weight

16742.11 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 86.3 %, Completeness (bb): 95.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.3 % (1520 of 1762)	90.5 % (830 of 917)	78.1 % (538 of 689)	97.4 % (152 of 156)
Backbone	95.7 % (848 of 886)	95.7 % (287 of 300)	95.3 % (422 of 443)	97.2 % (139 of 143)
Sidechain	79.0 % (805 of 1019)	87.4 % (539 of 617)	65.3 % (254 of 389)	92.3 % (12 of 13)
Aromatic	39.4 % (41 of 104)	75.0 % (39 of 52)	2.0 % (1 of 51)	100.0 % (1 of 1)
Methyl	93.0 % (173 of 186)	97.8 % (91 of 93)	88.2 % (82 of 93)

1. NeRF1

MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK MLADEFGTAS NIKSRVNRLS VLGAITSVQQ RLKLYNKVPP NGLVVYCGTI VTEEGKEKKV NIDFEPFKPI NTSLYLCDNK FHTEALTALL SDLEHHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	NeRF1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	25 mM
3	sodium phosphate	natural abundance	10 mM
4	beta-mercaptoethanol	natural abundance	2 mM
5	sodium azide	natural abundance	0.01 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
8	NeRF1	[U-99% 13C; U-99% 15N]	1 mM
9	sodium chloride	natural abundance	25 mM
10	sodium phosphate	natural abundance	10 mM
11	beta-mercaptoethanol	natural abundance	2 mM
12	sodium azide	natural abundance	0.01 %
13	D2O	natural abundance	100 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
14	NeRF1	natural abundance	1 mM
15	sodium chloride	natural abundance	25 mM
16	sodium phosphate	natural abundance	10 mM
17	beta-mercaptoethanol	natural abundance	2 mM
18	sodium azide	natural abundance	0.01 %
19	D2O	natural abundance	100 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
20	NeRF1	[U-99% 15N]	1 mM
21	sodium chloride	natural abundance	25 mM
22	sodium phosphate	natural abundance	10 mM
23	beta-mercaptoethanol	natural abundance	2 mM
24	sodium azide	natural abundance	0.01 %
25	H2O	natural abundance	90 %
26	D2O	natural abundance	10 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
27	NeRF1	[U-99% 15N]	1 mM
28	sodium chloride	natural abundance	25 mM
29	sodium phosphate	natural abundance	10 mM
30	beta-mercaptoethanol	natural abundance	2 mM
31	sodium azide	natural abundance	0.01 %
32	C12E5	natural abundance	5.00 %
33	hexanol	natural abundance	1.068 %
34	H2O	natural abundance	90 %
35	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LLX, Strand ID: A Detail

Heteronucl. T₁

125 T₁ values in 1 lists
Coherence Iz, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 6.8 Detail

Heteronucl. T₂

125 T₂ values in 1 lists
Coherence I(+,-), Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 6.8 Detail

Heteronucl. NOE

123 NOE values in 1 lists
Value type peak integral, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 6.8 Detail

Heteronucl. T₁/T₂

125 T₁/T₂ values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 6.8 Detail

Release date

2012-05-21

Citation

Structure and dynamics in solution of the stop codon decoding N-terminal domain of the human polypeptide chain release factor eRF1
Polshakov, V.I., Eliseev, B.D., Birdsall, B., Frolova, L.
Protein Sci. (2012), 21, 896-903, PubMed 22517631 , DOI 10.1002/pro.2067 , Abstract

Related entities 1. NeRF1, : 1 : 21 : 84 entities Detail

Interaction partners 1. NeRF1, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
20	NeRF1	[U-99% 15N]	1 mM
21	sodium chloride	natural abundance	25 mM
22	sodium phosphate	natural abundance	10 mM
23	beta-mercaptoethanol	natural abundance	2 mM
24	sodium azide	natural abundance	0.01 %
25	H2O	natural abundance	90 %
26	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	NeRF1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	25 mM
3	sodium phosphate	natural abundance	10 mM
4	beta-mercaptoethanol	natural abundance	2 mM
5	sodium azide	natural abundance	0.01 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

3 2D DQF-COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
14	NeRF1	natural abundance	1 mM
15	sodium chloride	natural abundance	25 mM
16	sodium phosphate	natural abundance	10 mM
17	beta-mercaptoethanol	natural abundance	2 mM
18	sodium azide	natural abundance	0.01 %
19	D2O	natural abundance	100 %

4 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
14	NeRF1	natural abundance	1 mM
15	sodium chloride	natural abundance	25 mM
16	sodium phosphate	natural abundance	10 mM
17	beta-mercaptoethanol	natural abundance	2 mM
18	sodium azide	natural abundance	0.01 %
19	D2O	natural abundance	100 %

5 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	NeRF1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	25 mM
3	sodium phosphate	natural abundance	10 mM
4	beta-mercaptoethanol	natural abundance	2 mM
5	sodium azide	natural abundance	0.01 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

6 3D HNCO

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	NeRF1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	25 mM
3	sodium phosphate	natural abundance	10 mM
4	beta-mercaptoethanol	natural abundance	2 mM
5	sodium azide	natural abundance	0.01 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

7 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	NeRF1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	25 mM
3	sodium phosphate	natural abundance	10 mM
4	beta-mercaptoethanol	natural abundance	2 mM
5	sodium azide	natural abundance	0.01 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

8 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	NeRF1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	25 mM
3	sodium phosphate	natural abundance	10 mM
4	beta-mercaptoethanol	natural abundance	2 mM
5	sodium azide	natural abundance	0.01 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

9 3D HNHA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
20	NeRF1	[U-99% 15N]	1 mM
21	sodium chloride	natural abundance	25 mM
22	sodium phosphate	natural abundance	10 mM
23	beta-mercaptoethanol	natural abundance	2 mM
24	sodium azide	natural abundance	0.01 %
25	H2O	natural abundance	90 %
26	D2O	natural abundance	10 %

10 3D HNHB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
20	NeRF1	[U-99% 15N]	1 mM
21	sodium chloride	natural abundance	25 mM
22	sodium phosphate	natural abundance	10 mM
23	beta-mercaptoethanol	natural abundance	2 mM
24	sodium azide	natural abundance	0.01 %
25	H2O	natural abundance	90 %
26	D2O	natural abundance	10 %

11 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	NeRF1	[U-99% 13C; U-99% 15N]	1 mM
2	sodium chloride	natural abundance	25 mM
3	sodium phosphate	natural abundance	10 mM
4	beta-mercaptoethanol	natural abundance	2 mM
5	sodium azide	natural abundance	0.01 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

12 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
8	NeRF1	[U-99% 13C; U-99% 15N]	1 mM
9	sodium chloride	natural abundance	25 mM
10	sodium phosphate	natural abundance	10 mM
11	beta-mercaptoethanol	natural abundance	2 mM
12	sodium azide	natural abundance	0.01 %
13	D2O	natural abundance	100 %

13 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
20	NeRF1	[U-99% 15N]	1 mM
21	sodium chloride	natural abundance	25 mM
22	sodium phosphate	natural abundance	10 mM
23	beta-mercaptoethanol	natural abundance	2 mM
24	sodium azide	natural abundance	0.01 %
25	H2O	natural abundance	90 %
26	D2O	natural abundance	10 %

14 3D 1H-13C NOESY

Instrument

Varian INOVA - 800 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
8	NeRF1	[U-99% 13C; U-99% 15N]	1 mM
9	sodium chloride	natural abundance	25 mM
10	sodium phosphate	natural abundance	10 mM
11	beta-mercaptoethanol	natural abundance	2 mM
12	sodium azide	natural abundance	0.01 %
13	D2O	natural abundance	100 %

15 2D IPAP

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
27	NeRF1	[U-99% 15N]	1 mM
28	sodium chloride	natural abundance	25 mM
29	sodium phosphate	natural abundance	10 mM
30	beta-mercaptoethanol	natural abundance	2 mM
31	sodium azide	natural abundance	0.01 %
32	C12E5	natural abundance	5.00 %
33	hexanol	natural abundance	1.068 %
34	H2O	natural abundance	90 %
35	D2O	natural abundance	10 %

16 2D J-modulated 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
20	NeRF1	[U-99% 15N]	1 mM
21	sodium chloride	natural abundance	25 mM
22	sodium phosphate	natural abundance	10 mM
23	beta-mercaptoethanol	natural abundance	2 mM
24	sodium azide	natural abundance	0.01 %
25	H2O	natural abundance	90 %
26	D2O	natural abundance	10 %

17 15N{1H} NOE

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
20	NeRF1	[U-99% 15N]	1 mM
21	sodium chloride	natural abundance	25 mM
22	sodium phosphate	natural abundance	10 mM
23	beta-mercaptoethanol	natural abundance	2 mM
24	sodium azide	natural abundance	0.01 %
25	H2O	natural abundance	90 %
26	D2O	natural abundance	10 %

18 15N T1

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
20	NeRF1	[U-99% 15N]	1 mM
21	sodium chloride	natural abundance	25 mM
22	sodium phosphate	natural abundance	10 mM
23	beta-mercaptoethanol	natural abundance	2 mM
24	sodium azide	natural abundance	0.01 %
25	H2O	natural abundance	90 %
26	D2O	natural abundance	10 %

19 15N T2

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
20	NeRF1	[U-99% 15N]	1 mM
21	sodium chloride	natural abundance	25 mM
22	sodium phosphate	natural abundance	10 mM
23	beta-mercaptoethanol	natural abundance	2 mM
24	sodium azide	natural abundance	0.01 %
25	H2O	natural abundance	90 %
26	D2O	natural abundance	10 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_18092_2llx.nef
Input source #2: Coordindates	2llx.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MADDPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MADDPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTI

-------110-------120-------130-------140-------150
VTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDLEHHHHHH
||||||||||||||||||||||||||||||||||||||||||||||||||
VTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDLEHHHHHH

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	150	0	0	100.0

Content subtype: combined_18092_2llx.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1511		97.3 (chain: A, length: 150)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	2951 (1)	noe	94.7 (chain: A, length: 150)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	38 (1)	hbond	13.3 (chain: A, length: 150)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	Warning	316 (316)	.	94.7 (chain: A, length: 150)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_5	OK	69 (69)	.	46.0 (chain: A, length: 150)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 150, Sequence coverage: 97.3%
- Total number of assignments
  - ¹H chemical shifts: 837
  - ¹³C chemical shifts: 522
  - ¹⁵N chemical shifts: 152
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	917	837	91.3
¹³C chemical shifts	689	522	75.8
¹⁵N chemical shifts	162	152	93.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	300	291	97.0
¹³C chemical shifts	300	281	93.7
¹⁵N chemical shifts	143	138	96.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	617	546	88.5
¹³C chemical shifts	389	241	62.0
¹⁵N chemical shifts	19	14	73.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	96	93	96.9
¹³C chemical shifts	96	84	87.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	52	38	73.1
¹³C chemical shifts	51	1	2.0
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 150, Sequence coverage: 94.7%
- Total number of restraints: 2943
- Weight of restraints: 1.0 (2943)
- Potential type of restraints: square-well-parabolic (2943)
- Range of distance target values: 1.25, 3.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: Warning
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 150, Sequence coverage: 13.3%
  - Total number of restraints: 38
  - Weight of restraints: 1.0 (38)
  - Potential type of restraints: square-well-parabolic (38)
  - Range of distance target values: 1.9, 2.95 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: Warning
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 150, Sequence coverage: 94.7%
- Total number of restraints: 316
- Total number of restraints per polymer type: 316
- Weight of restraints: 1.0 (316)
- Potential type of restraints: square-well-parabolic (316)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_5
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 150, Sequence coverage: 46.0%
- Total number of restraints: 69
- Potential type of restraints: undefined (69)
- Range of observed RDC values: 100.0, -100.0 (Hz)
- RDC restraints per residue

Keywords protein structure and dynamics, stop codon recognition, termination of protein synthesis

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Found 1 Document (1.143 seconds)

BMRB: 18092

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. NeRF1, : 1 : 21 : 84 entities Detail

Interaction partners 1. NeRF1, : 1 interactors Detail

Experiments performed 19 experiments Detail

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NMR combined restraints 6 contents Detail