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BMRB: 18165

2LNH

Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly

Authors

Aitio, O., Hellman, M., Skehan, B., Kesti, T., Leong, J.M., Saksela, K., Permi, P.

Assembly

Enterohaemorrhagic E. coli (EHEC)

Entity

1. Enterohaemorrhagic E. coli (EHEC), entity 1 (polymer, Thiol state: all free), 65 monomers, 7253.957 Da Detail

GSNFQHIGHV GWDPNTGFDL NNLDPELKNL FDMCGISEAQ LKDRETSKVI YDFIEKTGGV EAVKN

2. Enterohaemorrhagic E. coli (EHEC), entity 2 (polymer, Thiol state: not present), 67 monomers, 7547.509 Da Detail

GSHMKKQKVK TIFPHTAGSN KTLLSFAQGD VITLLIPEEK DGWLYGEHDV SKARGWFPSS YTKLLEE

3. Enterohaemorrhagic E. coli (EHEC), entity 3 (polymer, Thiol state: not present), 48 monomers, 5295.919 Da Detail

GLPDVAQRLM QHLAEHGIQP ARNMAEHIPP APNWPAPTPP VQNEQSRP

Total weight

20097.385 Da

Max. entity weight

7547.509 Da

Source organism

Homo sapiens , Escherichia coli

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 83.9 %, Completeness (bb): 79.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.9 % (1768 of 2108)	90.5 % (999 of 1104)	74.5 % (608 of 816)	85.6 % (161 of 188)
Backbone	79.5 % (835 of 1050)	92.2 % (332 of 360)	67.2 % (353 of 525)	90.9 % (150 of 165)
Sidechain	89.5 % (1094 of 1223)	89.7 % (667 of 744)	91.2 % (416 of 456)	47.8 % (11 of 23)
Aromatic	86.2 % (150 of 174)	96.6 % (84 of 87)	74.7 % (62 of 83)	100.0 % (4 of 4)
Methyl	98.8 % (170 of 172)	98.8 % (85 of 86)	98.8 % (85 of 86)

1. entity 1

GSNFQHIGHV GWDPNTGFDL NNLDPELKNL FDMCGISEAQ LKDRETSKVI YDFIEKTGGV EAVKN

2. entity 2

GSHMKKQKVK TIFPHTAGSN KTLLSFAQGD VITLLIPEEK DGWLYGEHDV SKARGWFPSS YTKLLEE

3. entity 3

GLPDVAQRLM QHLAEHGIQP ARNMAEHIPP APNWPAPTPP VQNEQSRP

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	entity_1	[U-98% 13C; U-98% 15N]	0.3 mM
2	entity_2	natural abundance	0.3 mM
3	entity_3	natural abundance	0.3 mM
4	H2O	natural abundance	93 %
5	D2O	natural abundance	7 %
6	Na-PO4	natural abundance	20 mM
7	NaCl	natural abundance	50 mM

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
8	entity_1	natural abundance	0.5 mM
9	entity_2	[U-98% 13C; U-98% 15N]	0.5 mM
10	entity_3	natural abundance	0.5 mM
11	H2O	natural abundance	93 %
12	D2O	natural abundance	7 %
13	Na-PO4	natural abundance	20 mM
14	NaCl	natural abundance	50 mM

Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
15	entity_1	natural abundance	0.5 mM
16	entity_2	natural abundance	0.5 mM
17	entity_3	[U-98% 13C; U-98% 15N]	0.5 mM
18	H2O	natural abundance	93 %
19	D2O	natural abundance	7 %
20	Na-PO4	natural abundance	20 mM
21	NaCl	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.783 ppm	internal	indirect	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.94 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.783 ppm	internal	indirect	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.94 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.783 ppm	internal	indirect	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LNH, Strand ID: A, B, C Detail

Release date

2012-08-26

Citation

Enterohaemorrhagic Escherichia coli exploits a tryptophan switch to hijack host f-actin assembly
Aitio, O., Hellman, M., Skehan, B., Kesti, T., Leong, J.M., Saksela, K., Permi, P.
Structure (2012), 20, 1692-1703, PubMed 22921828 , DOI 10.1016/j.str.2012.07.015 , Abstract

Entries sharing articles Swiss-Prot: 3 entries Detail

  Swiss-Prot: P0DJ89 released on 2011-11-16
    Title ESFU3_ECO57 Entity Secreted effector protein EspF(U)
  Swiss-Prot: Q9UHR4 released on 2006-07-25
    Title BI2L1_HUMAN Entity Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1
  Swiss-Prot: O00401 released on 2001-04-27
    Title WASL_HUMAN Entity Neural Wiskott-Aldrich syndrome protein

Related entities 1. Enterohaemorrhagic E. coli (EHEC), entity 1, : 7 : 16 entities Detail

Related entities 2. Enterohaemorrhagic E. coli (EHEC), entity 2, : 2 : 139 entities Detail

Related entities 3. Enterohaemorrhagic E. coli (EHEC), entity 3, : 1 : 1 : 6 : 16 entities Detail

Interaction partners 1. Enterohaemorrhagic E. coli (EHEC), entity 1, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Interaction partners 2. Enterohaemorrhagic E. coli (EHEC), entity 2, : 34 interactors Detail

More details for 34 interactors identified by 8 citations

Experiments performed 30 experiments Detail

1 2D 1H-15N HSQC