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Solution structure of GspC-HR of typeII secretion system
Authors
Gu, S., Kelly, G., Pickersgill, R.
Assembly
GspC-HR of typeII secretion system
Entity
1. GspC-HR of typeII secretion system (polymer, Thiol state: not present), 104 monomers, 11129.22 Da Detail

GSHMLEMAGA LDASQMSNLP PSTLNLSLTG VMAGDDDSRS IAIISKDNEQ FSRGVNEEVP GYNAKIVSIR PDRVVLQYQG RYEVLGLYSQ EDSGSDGVPG AQVR


Formula weight
11129.22 Da
Source organism
Dickeya dadantii
Exptl. method
SOLUTION NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 65.4 %, Completeness: 61.8 %, Completeness (bb): 65.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All61.8 % (703 of 1137)61.8 % (363 of 587)61.3 % (266 of 434)63.8 % (74 of 116)
Backbone65.0 % (392 of 603)65.0 % (132 of 203)64.5 % (194 of 301)66.7 % (66 of 99)
Sidechain59.6 % (374 of 627)60.2 % (231 of 384)59.7 % (135 of 226)47.1 % (8 of 17)
Aromatic26.1 % (12 of 46)52.2 % (12 of 23) 0.0 % (0 of 23)
Methyl70.9 % (78 of 110)69.1 % (38 of 55)72.7 % (40 of 55)

1. entity

GSHMLEMAGA LDASQMSNLP PSTLNLSLTG VMAGDDDSRS IAIISKDNEQ FSRGVNEEVP GYNAKIVSIR PDRVVLQYQG RYEVLGLYSQ EDSGSDGVPG AQVR

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.0, Details 0.5mM 15N 13C OutC-HRF3 20mM Tis pH7.0


#NameIsotope labelingTypeConcentration
1OutC-HRF3[U-100% 13C; U-100% 15N]0.5 mM
2Tisnatural abundance20 mM
3D2Onatural abundance10 %
4H2Onatural abundance90 %

LACS Plot; CA
Referencing offset: 0.19 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.19 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.07 ppm, Outliers: 3 Detail
LACS Plot; CO
Referencing offset: 0.84 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 2LNV, Strand ID: A Detail


Release date
2012-03-08
Citation
Solution structure of homology region (HR) domain of type II secretion system
Gu, S., Kelly, G., Wang, X., Frenkiel, T., Shevchik, V.E., Pickersgill, R.W.
J. Biol. Chem. (2012), 287, 9072-9080, PubMed 22253442 , DOI 10.1074/jbc.M111.300624 ,
Related entities 1. GspC-HR of typeII secretion system, : 1 : 1 : 4 entities Detail
Experiments performed 12 experiments Detail
Keywords GspC, OutC