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BMRB: 18186

2LNZ

Solution structure of the Get5 carboxyl domain from S. cerevisiae

Authors

Chartron, J.W., VanderVelde, D.G., Rao, M., Clemons, W.M.

Assembly

Get5 carboxyl domain from S. cerevisiae

Entity

1. Get5 carboxyl domain from S. cerevisiae (polymer, Thiol state: not present), 64 monomers, 7116.833 × 2 Da Detail

SVDPTISKEP EAEKSTNSPA PAPPQELTVP WDDIEALLKN NFENDQAAVR QVMERLQKGW SLAK

Total weight

14233.666 Da

Max. entity weight

7116.833 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 93.7 %, Completeness (bb): 95.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.7 % (696 of 743)	91.6 % (359 of 392)	95.8 % (272 of 284)	97.0 % (65 of 67)
Backbone	95.7 % (354 of 370)	95.9 % (117 of 122)	95.3 % (182 of 191)	96.5 % (55 of 57)
Sidechain	92.7 % (404 of 436)	89.6 % (242 of 270)	97.4 % (152 of 156)	100.0 % (10 of 10)
Aromatic	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (15 of 15)	100.0 % (2 of 2)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. Get5

SVDPTISKEP EAEKSTNSPA PAPPQELTVP WDDIEALLKN NFENDQAAVR QVMERLQKGW SLAK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1

#	Name	Isotope labeling	Concentration
1	Get5	[U-100% 13C; U-100% 15N]	3.5 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium azide	natural abundance	0.02 %
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1

#	Name	Isotope labeling	Concentration
6	Get5	[U-100% 15N]	2.5 mM
7	sodium phosphate	natural abundance	20 mM
8	sodium azide	natural abundance	0.02 %
9	polyacrylamide	natural abundance	4 %
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.1

#	Name	Isotope labeling	Concentration
12	Get5	[U-100% 15N]	2.5 mM
13	sodium phosphate	natural abundance	20 mM
14	sodium azide	natural abundance	0.02 %
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.255145
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.255145
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.255145
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.255145
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LNZ, Strand ID: A, B Detail

Release date

2012-02-09

Citation

Get5 carboxyl-terminal domain is a novel dimerization motif that tethers an extended Get4/Get5 complex
Chartron, J.W., VanderVelde, D.G., Rao, M., Clemons, W.M.
J. Biol. Chem. (2012), 287, 8310-8317, PubMed 22262836 , DOI 10.1074/jbc.M111.333252 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18187 released on 2012-02-09
Title Solution structure of the Get5 carboxyl domain from A. fumigatus

Related entities 1. Get5 carboxyl domain from S. cerevisiae, : 1 : 4 entities Detail

Interaction partners 1. Get5 carboxyl domain from S. cerevisiae, : 15 interactors Detail

More details for 15 interactors identified by 5 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC