BMRB: 18276

1H, 13C and 15N chemical shifts for Salmonella Typhimurium PrgI Type three secretion system needle subunit

Authors

Loquet, A., Lv, G., Giller, K., Becker, S., Lange, A.

Assembly

PrgI needle

Entity

1. PrgI needle (polymer, Thiol state: all free), 80 monomers, 8856.801 Da Detail

MATPWSGYLD DVSAKFDTGV DNLQTQVTEA LDKLAAKPSD PALLAAYQSK LSEYNLYRNA QSNTVKVFKD IDAAIIQNFR

Formula weight

8856.801 Da

Source organism

Salmonella enterica

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 54.0 %, Completeness (bb): 81.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	54.0 % (497 of 921)	15.4 % (73 of 474)	96.9 % (348 of 359)	86.4 % (76 of 88)
Backbone	81.2 % (385 of 474)	45.9 % (73 of 159)	99.2 % (236 of 238)	98.7 % (76 of 77)
Sidechain	36.2 % (190 of 525)	0.0 % (0 of 315)	95.5 % (190 of 199)	0.0 % (0 of 11)
Aromatic	36.5 % (27 of 74)	0.0 % (0 of 37)	75.0 % (27 of 36)	0.0 % (0 of 1)
Methyl	50.0 % (48 of 96)	0.0 % (0 of 48)	100.0 % (48 of 48)

1. PrgI

MATPWSGYLD DVSAKFDTGV DNLQTQVTEA LDKLAAKPSD PALLAAYQSK LSEYNLYRNA QSNTVKVFKD IDAAIIQNFR

Show sample condition

Sample

Solvent system wet, Pressure 1 atm, Temperature 278 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	PrgI	[U-100% 13C; U-100% 15N]		10 mg

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Protein Blocks Logo

Calculated from 15 models in PDB: 2MEX, Strand ID: A, B, C, D Detail

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Release date

2012-03-22

Citation

13C spin dilution for simplified and complete solid-state NMR resonance assignment of insoluble biological assemblies
Loquet, A., Lv, G., Giller, K., Becker, S., Lange, A.
J. Am. Chem. Soc. (2011), 133, 4722-4725, PubMed 21401039 , DOI 10.1021/ja200066s , Abstract

Related entities 1. PrgI needle, : 1 : 23 : 2 : 4 : 16 entities Detail

Interaction partners 1. PrgI needle, : 3 interactors Detail

Experiments performed 2 experiments Detail