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Characterization of the chromoshadow domain-mediated binding of heterochromatin protein 1 alpha (HP1 alpha) to histone H3: Backbone 1H, 15N chemical shift assignments for histone H3 (1-59)
Authors
Richart, A.N., Clair, B.I., Katherine, S., Natalia, M.V., Jean, T.O.
Assembly
H3(1-59)
Entity
1. H3(1-59) (polymer, Thiol state: not present), 59 monomers, 6503.444 Da Detail

ARTKQTARKS TGGKAPRKQL ATKAARKSAP ATGGVKKPHR YRPGTVALRE IRRYQKSTE


Formula weight
6503.444 Da
Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 93.2 %, Completeness: 28.4 %, Completeness (bb): 63.9 % Detail

Polymer type: polypeptide(L)

Total1H15N
All28.4 % (124 of 437)18.6 % (69 of 370)82.1 % (55 of 67)
Backbone63.9 % (108 of 169)46.5 % (53 of 114)100.0 % (55 of 55)
Sidechain 6.0 % (16 of 268) 6.3 % (16 of 256) 0.0 % (0 of 12)
Aromatic 0.0 % (0 of 10) 0.0 % (0 of 10)
Methyl 7.7 % (2 of 26) 7.7 % (2 of 26)

1. H3(1-59)

ARTKQTARKS TGGKAPRKQL ATKAARKSAP ATGGVKKPHR YRPGTVALRE IRRYQKSTE

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0


#NameIsotope labelingTypeConcentration
1H3(1-59)[U-95% 15N]0.5 mM
2sodium phosphatenatural abundance10 mM
3H2Onatural abundance90 %
4D2Onatural abundance10 %

Release date
2012-05-20
Citation
Characterization of chromoshadow domain-mediated binding of heterochromatin protein 1α (HP1α) to histone H3
Richart, A.N., Brunner, C.I.W., Stott, K., Murzina, N.V., Thomas, J.O.
J. Biol. Chem. (2012), 287, 18730-18737, PubMed 22493481 , DOI 10.1074/jbc.M111.337204 ,
Related entities 1. H3(1-59), : 1 : 104 : 17 : 6 : 54 entities Detail
Interaction partners 1. H3(1-59), : 22 interactors Detail
Experiments performed 3 experiments Detail
Keywords chromatin, chromoshadow domain, H3 alpha-N helix, histone H3, HP1, NMR