BMRB: 18385

Characterization of the chromoshadow domain-mediated binding of heterochromatin protein 1 alpha (HP1 alpha) to histone H3: Backbone 1H, 15N chemical shift assignments for histone H3 (1-59)

Authors

Richart, A.N., Clair, B.I., Katherine, S., Natalia, M.V., Jean, T.O.

Assembly

H3(1-59)

Entity

1. H3(1-59) (polymer, Thiol state: not present), 59 monomers, 6503.444 Da Detail

ARTKQTARKS TGGKAPRKQL ATKAARKSAP ATGGVKKPHR YRPGTVALRE IRRYQKSTE

Formula weight

6503.444 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.2 %, Completeness: 29.9 %, Completeness (bb): 65.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	29.9 % (128 of 428)	19.7 % (73 of 370)	94.8 % (55 of 58)
Backbone	65.5 % (114 of 174)	49.6 % (59 of 119)	100.0 % (55 of 55)
Sidechain	5.5 % (14 of 254)	5.6 % (14 of 251)	0.0 % (0 of 3)
Aromatic	0.0 % (0 of 10)	0.0 % (0 of 10)
Methyl	7.7 % (2 of 26)	7.7 % (2 of 26)

1. H3(1-59)

ARTKQTARKS TGGKAPRKQL ATKAARKSAP ATGGVKKPHR YRPGTVALRE IRRYQKSTE

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	H3(1-59)	[U-95% 15N]		0.5 mM
2	sodium phosphate	natural abundance		10 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

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Release date

2012-05-20

Citation

Characterization of chromoshadow domain-mediated binding of heterochromatin protein 1α (HP1α) to histone H3
Richart, A.N., Brunner, C.I.W., Stott, K., Murzina, N.V., Thomas, J.O.
J. Biol. Chem. (2012), 287, 18730-18737, PubMed 22493481 , DOI 10.1074/jbc.M111.337204 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. H3(1-59), : 1 : 116 : 34 : 9 : 100 entities Detail

Interaction partners 1. H3(1-59), : 18 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail