BMRBj - BMREntryMaster

Found 1 Document (1.231 seconds)

BMRB: 18582

2LVO

1H, 13C, 15N chemical shifts of gp78CUE bound to ubiquitin AND backbone amide shifts of ubiquitin bound to gp78CUE

Authors

Liu, S., Chen, Y., Huang, T., Tarasov, S.G., King, A., Li, J., Weissman, A.M., Byrd, R.A., Das, R.

Assembly

gp78CUE bound to ubiquitin

Entity

1. ubiquitin (polymer, Thiol state: not present), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

2. gp78CUE (polymer), 52 monomers, 5962.717 Da Detail

SNSQLNAMAH QIQEMFPQVP YHLVLQDLQL TRSVEITTDN ILEGRIQVPF PT

Total weight

14527.448 Da

Max. entity weight

8564.731 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 50.8 %, Completeness (bb): 59.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	50.8 % (779 of 1533)	50.7 % (407 of 803)	42.1 % (249 of 591)	88.5 % (123 of 139)
Backbone	59.0 % (445 of 754)	68.0 % (174 of 256)	41.4 % (156 of 377)	95.0 % (115 of 121)
Sidechain	42.9 % (386 of 900)	42.8 % (234 of 547)	43.0 % (144 of 335)	44.4 % (8 of 18)
Aromatic	52.9 % (36 of 68)	52.9 % (18 of 34)	52.9 % (18 of 34)
Methyl	44.6 % (74 of 166)	45.8 % (38 of 83)	43.4 % (36 of 83)

1. ubiquitin

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

2. gp78CUE

SNSQLNAMAH QIQEMFPQVP YHLVLQDLQL TRSVEITTDN ILEGRIQVPF PT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 50 mM Tris pH 7.2 50mM NaCl

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 mM
2	sodium chloride	natural abundance	50 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.75 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LVO, Strand ID: A, C Detail

Release date

2012-11-18

Citation

Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains
Liu, S., Chen, Y., Li, J., Huang, T., Tarasov, S., King, A., Weissman, A.M., Byrd, R., Das, R.
Structure (2012), 20, 2138-2150, PubMed 23123110 , DOI 10.1016/j.str.2012.09.020 , Abstract

Related entities 1. ubiquitin, : 92 : 6 : 46 : 182 entities Detail

Related entities 2. gp78CUE, : 1 : 5 : 2 : 2 : 24 entities Detail

Interaction partners 2. gp78CUE, : 33 interactors Detail

More details for 33 interactors identified by 11 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC