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BMRB: 18584

2LVQ

Solution structure of gp78CUE/K48-Ub2 complex

Authors

Liu, S., Chen, Y., Huang, T., Tarasov, S.G., King, A., Li, J., Weissman, A.M., Byrd, R.A., Das, R.

Assembly

gp78CUE/K48-Ub2 complex

Entity

1. ubiquitin (polymer), 76 monomers, 8564.731 × 2 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

2. gp78CUE (polymer), 52 monomers, 5962.717 Da Detail

SNSQLNAMAH QIQEMFPQVP YHLVLQDLQL TRSVEITTDN ILEGRIQVPF PT

Total weight

23092.18 Da

Max. entity weight

8564.731 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 38.3 %, Completeness: 10.9 %, Completeness (bb): 26.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	10.9 % (103 of 942)	6.6 % (53 of 803)	36.0 % (50 of 139)
Backbone	26.0 % (98 of 377)	19.1 % (49 of 256)	40.5 % (49 of 121)
Sidechain	0.9 % (5 of 565)	0.7 % (4 of 547)	5.6 % (1 of 18)
Aromatic	0.0 % (0 of 34)	0.0 % (0 of 34)
Methyl	0.0 % (0 of 83)	0.0 % (0 of 83)

1. ubiquitin

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

2. gp78CUE

SNSQLNAMAH QIQEMFPQVP YHLVLQDLQL TRSVEITTDN ILEGRIQVPF PT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 50 mM Tris pH 7.2 50mM NaCl

#	Name	Isotope labeling	Concentration
1	ubiquitin		0.0 ~ 0.0 mM
2	gp78CUE		0.0 ~ 0.0 mM
3	TRIS	natural abundance	50 mM
4	sodium chloride	natural abundance	50 mM

Chem. Shift Complete2

Sequence coverage: 52.3 %, Completeness: 12.6 %, Completeness (bb): 30.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	12.6 % (238 of 1884)	7.5 % (121 of 1606)	42.1 % (117 of 278)
Backbone	30.9 % (233 of 754)	22.9 % (117 of 512)	47.9 % (116 of 242)
Sidechain	0.5 % (6 of 1130)	0.5 % (5 of 1094)	2.8 % (1 of 36)
Aromatic	0.0 % (0 of 68)	0.0 % (0 of 68)
Methyl	0.0 % (0 of 166)	0.0 % (0 of 166)

1. ubiquitin

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

2. gp78CUE

SNSQLNAMAH QIQEMFPQVP YHLVLQDLQL TRSVEITTDN ILEGRIQVPF PT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 50 mM Tris pH 7.2 50mM NaCl

#	Name	Isotope labeling	Concentration
1	ubiquitin		0.0 ~ 0.0 mM
2	gp78CUE		0.0 ~ 0.0 mM
3	TRIS	natural abundance	50 mM
4	sodium chloride	natural abundance	50 mM

Protein Blocks Logo

Calculated from 24 models in PDB: 2LVQ, Strand ID: A, B, D Detail

Release date

2012-11-18

Citation

Promiscuous interactions of gp78 E3 ligase CUE domain with polyubiquitin chains
Liu, S., Chen, Y., Li, J., Huang, T., Tarasov, S., King, A., Weissman, A.M., Byrd, R., Das, R.
Structure (2012), 20, 2138-2150, PubMed 23123110 , DOI 10.1016/j.str.2012.09.020 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 18581 released on 2012-11-18
    Title Solution structure of gp78 CUE domain
  BMRB: 18582 released on 2012-11-18
    Title 1H, 13C, 15N chemical shifts of gp78CUE bound to ubiquitin AND backbone amide shifts of ubiquitin bound to gp78CUE
  BMRB: 18583 released on 2012-11-18
    Title Solution structure of the gp78CUE/K48-Ub2 complex

Related entities 1. ubiquitin, : 92 : 6 : 47 : 182 entities Detail

Related entities 2. gp78CUE, : 1 : 5 : 2 : 2 : 24 entities Detail

Interaction partners 2. gp78CUE, : 33 interactors Detail

More details for 33 interactors identified by 11 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC