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BMRB: 18665

1Q5H

Human dUTPase

Authors

Isaksson, J., Woestenenk, E., Sahlberg, C., Agback, T.

Assembly

dUTPase homotrimer

Entity

1. dUTPase homotrimer (polymer, Thiol state: all free), 148 monomers, 16349.25 × 3 Da Detail

MHHHHHHMQL RFARLSEHAT APTRGSARAA GYDLYSAYDY TIPPMEKAVV KTDIQIALPS GCYGRVAPRS GLAAKHFIDV GAGVIDEDYR GNVGVVLFNF GKEKFEVKKG DRIAQLICER IFYPEIEEVQ ALDDTERGSG GFGSTGKN

Total weight

49047.75 Da

Max. entity weight

16349.25 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.8 %, Completeness: 37.3 %, Completeness (bb): 66.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	37.3 % (625 of 1677)	19.1 % (166 of 868)	51.2 % (338 of 660)	81.2 % (121 of 149)
Backbone	66.9 % (586 of 876)	42.5 % (130 of 306)	78.3 % (335 of 428)	85.2 % (121 of 142)
Sidechain	14.9 % (139 of 933)	6.0 % (34 of 562)	28.8 % (105 of 364)	0.0 % (0 of 7)
Aromatic	2.5 % (4 of 158)	5.1 % (4 of 79)	0.0 % (0 of 79)
Methyl	11.3 % (17 of 150)	6.7 % (5 of 75)	16.0 % (12 of 75)

1. dUTPase

MHHHHHHMQL RFARLSEHAT APTRGSARAA GYDLYSAYDY TIPPMEKAVV KTDIQIALPS GCYGRVAPRS GLAAKHFIDV GAGVIDEDYR GNVGVVLFNF GKEKFEVKKG DRIAQLICER IFYPEIEEVQ ALDDTERGSG GFGSTGKN

Show sample condition

Sample #1

Solvent system 50% H2O/50% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details Apo form

#	Name	Isotope labeling	Concentration
1	dUTPase	[U-15N; U-13C; U-50% 2H]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium azide	natural abundance	0.01 %
4	sodium chloride	natural abundance	50 mM
5	DTT	[U-2H]	1 mM
6	D2O	[U-2H]	10 %

Sample #2

Solvent system 50% H2O/50% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details Complex with dUPNPP

#	Name	Isotope labeling	Concentration
7	dUTPase	[U-15N; U-13C; U-50% 2H]	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium azide	natural abundance	0.01 %
10	sodium chloride	natural abundance	50 mM
11	DTT	[U-2H]	1 mM
12	D2O	[U-2H]	10 %
13	2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE	natural abundance	1.2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.79 ppm	internal	indirect	0.2514914
¹H	water	protons	4.79 ppm	internal	direct	1.0
¹⁵N	water	protons	4.79 ppm	internal	indirect	0.1013404

Referencing offset: 0.95 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.79 ppm	internal	indirect	0.2514914
¹H	water	protons	4.79 ppm	internal	direct	1.0
¹⁵N	water	protons	4.79 ppm	internal	indirect	0.1013404

Referencing offset: 0.95 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.79 ppm	internal	indirect	0.2514914
¹H	water	protons	4.79 ppm	internal	direct	1.0
¹⁵N	water	protons	4.79 ppm	internal	indirect	0.1013404

Referencing offset: 0.82 ppm, Outliers: 2 Detail

Release date

2013-02-11

Citation

Backbone nuclear magnetic resonance assignment of human deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase)
Isaksson, J., Woestenenk, E., Sahlberg, C., Agback, T.
Biomol. NMR Assign. (2014), 8, 81-84, PubMed 23307478 , DOI 10.1007/s12104-013-9457-7 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18666 released on 2013-02-11
Title Human dUTPase

Related entities 1. dUTPase homotrimer, : 1 : 8 : 201 entities Detail

Interaction partners 1. dUTPase homotrimer, : 24 interactors Detail

More details for 24 interactors identified by 9 citations