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BMRB: 18666

1Q5H

Human dUTPase

Authors

Isaksson, J., Woestenenk, E., Sahlberg, C., Agback, T.

Assembly

dUTPase homotrimer

Entity

1. dUTPase (polymer, Thiol state: all free), 148 monomers, 16349.25 × 3 Da Detail

MHHHHHHMQL RFARLSEHAT APTRGSARAA GYDLYSAYDY TIPPMEKAVV KTDIQIALPS GCYGRVAPRS GLAAKHFIDV GAGVIDEDYR GNVGVVLFNF GKEKFEVKKG DRIAQLICER IFYPEIEEVQ ALDDTERGSG GFGSTGKN

2. dUTPase homotrimer, entity DUP (non-polymer), 467.157 Da

Total weight

49514.906 Da

Max. entity weight

16349.25 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.1 %, Completeness: 36.9 %, Completeness (bb): 68.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	36.9 % (618 of 1677)	16.6 % (144 of 868)	53.6 % (354 of 660)	80.5 % (120 of 149)
Backbone	68.0 % (596 of 876)	41.8 % (128 of 306)	81.3 % (348 of 428)	84.5 % (120 of 142)
Sidechain	13.9 % (130 of 933)	2.8 % (16 of 562)	31.3 % (114 of 364)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 158)	0.0 % (0 of 79)	0.0 % (0 of 79)
Methyl	14.7 % (22 of 150)	5.3 % (4 of 75)	24.0 % (18 of 75)

1. dUTPase

MHHHHHHMQL RFARLSEHAT APTRGSARAA GYDLYSAYDY TIPPMEKAVV KTDIQIALPS GCYGRVAPRS GLAAKHFIDV GAGVIDEDYR GNVGVVLFNF GKEKFEVKKG DRIAQLICER IFYPEIEEVQ ALDDTERGSG GFGSTGKN

Show sample condition

Sample #1

Solvent system 50% H2O/50% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details Apo form

#	Name	Isotope labeling	Concentration
1	dUTPase	[U-15N; U-13C; U-50% 2H]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium azide	natural abundance	0.01 %
4	sodium chloride	natural abundance	50 mM
5	DTT	[U-2H]	1 mM
6	D2O	[U-2H]	10 %

Sample #2

Solvent system 50% H2O/50% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details Complex with dUPNPP

#	Name	Isotope labeling	Concentration
7	dUTPase	[U-15N; U-13C; U-50% 2H]	1 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium azide	natural abundance	0.01 %
10	sodium chloride	natural abundance	50 mM
11	DTT	[U-2H]	1 mM
12	D2O	[U-2H]	10 %
13	2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE	natural abundance	1.2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.79 ppm	internal	indirect	0.2514914
¹H	water	protons	4.79 ppm	internal	direct	1.0
¹⁵N	water	protons	4.79 ppm	internal	indirect	0.1013404

Referencing offset: 1.13 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.79 ppm	internal	indirect	0.2514914
¹H	water	protons	4.79 ppm	internal	direct	1.0
¹⁵N	water	protons	4.79 ppm	internal	indirect	0.1013404

Referencing offset: 1.13 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.79 ppm	internal	indirect	0.2514914
¹H	water	protons	4.79 ppm	internal	direct	1.0
¹⁵N	water	protons	4.79 ppm	internal	indirect	0.1013404

Referencing offset: 0.74 ppm, Outliers: 2 Detail

Release date

2013-02-11

Citation

Backbone nuclear magnetic resonance assignment of human deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase)
Isaksson, J., Woestenenk, E., Sahlberg, C., Agback, T.
Biomol. NMR Assign. (2014), 8, 81-84, PubMed 23307478 , DOI 10.1007/s12104-013-9457-7 , Abstract

Related entities 1. dUTPase, : 1 : 8 : 201 entities Detail

Interaction partners 1. dUTPase, : 24 interactors Detail

More details for 24 interactors identified by 9 citations