BMRB: 18688

Backbone amide chemical shifts of gp78 RING bound to Ube2g2:G2BR

Authors

Das, R., Linag, Y., Mariano, J., Li, J., Huang, T., King, A., Weissman, A., Ji, X., Byrd, R.

Assembly

gp78 RING bound to Ube2g2:G2BR

Entity

1. gp78 RING bound to Ube2g2:G2BR, entity 1 (polymer, Thiol state: all free), 156 monomers, 17540.01 Da Detail

AGTALKRLMA EYKQLTLNPP EGIVAGPMNE ENFFEWEALI MGPEDTCFEF GVFPAILSFP LDYPLSPPKM RFTCEMFHPN IYPDGRVCIS ILHAPGSAER WSPVQSVEKI LLSVVSMLAE PNDESGANVD ASKMWRDDRE QFYKIAKQIV QKSLGL

2. gp78 RING bound to Ube2g2:G2BR, entity 2 (polymer, Thiol state: not present), 27 monomers, 3357.844 Da Detail

SADERQRMLV QRKDELLQQA RKRFLNK

3. gp78 RING bound to Ube2g2:G2BR, entity 3 (polymer, Thiol state: all other bound), 58 monomers, 6467.308 Da Detail

AVATPEELAV NNDDCAICWD SMQAARKLPC GHLFHNSCLR SWLEQDTSCP TCRMSLNI

4. gp78 RING bound to Ube2g2:G2BR, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

27495.98 Da

Max. entity weight

17540.01 Da

Entity Connection

metal coordination 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	3:HIS32:ND1	4:ZN1:ZN
2	metal coordination	sing	3:CYS52:SG	4:ZN1:ZN
3	metal coordination	sing	3:CYS49:SG	4:ZN1:ZN
4	metal coordination	sing	3:CYS30:SG	4:ZN1:ZN
5	metal coordination	sing	3:HIS35:ND1	4:ZN1:ZN
6	metal coordination	sing	3:CYS18:SG	4:ZN1:ZN
7	metal coordination	sing	3:CYS38:SG	4:ZN1:ZN
8	metal coordination	sing	3:CYS15:SG	4:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 25.3 %, Completeness: 8.1 %, Completeness (bb): 18.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	8.1 % (140 of 1738)	5.4 % (80 of 1487)	23.9 % (60 of 251)
Backbone	18.2 % (127 of 699)	14.1 % (67 of 475)	26.8 % (60 of 224)
Sidechain	1.3 % (13 of 1039)	1.3 % (13 of 1012)	0.0 % (0 of 27)
Aromatic	0.0 % (0 of 114)	0.0 % (0 of 109)	0.0 % (0 of 5)
Methyl	0.0 % (0 of 121)	0.0 % (0 of 121)

1. entity 1

AGTALKRLMA EYKQLTLNPP EGIVAGPMNE ENFFEWEALI MGPEDTCFEF GVFPAILSFP LDYPLSPPKM RFTCEMFHPN IYPDGRVCIS ILHAPGSAER WSPVQSVEKI LLSVVSMLAE PNDESGANVD ASKMWRDDRE QFYKIAKQIV QKSLGL

2. entity 2

SADERQRMLV QRKDELLQQA RKRFLNK

3. entity 3

AVATPEELAV NNDDCAICWD SMQAARKLPC GHLFHNSCLR SWLEQDTSCP TCRMSLNI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	gp78RING	[U-100% 13C; U-100% 15N]		0.7 ~ 1.0 mM
2	TRIS	natural abundance		50 mM
3	TCEP	natural abundance		2 mM
4	sodium azide	natural abundance		0.2 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2LXP, Strand ID: A, B, C Detail

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Release date

2013-08-25

Citation

Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine
Das, R., Liang, Y., Mariano, J., Li, J., Huang, T., King, A., Tarasov, S.G., Weissman, A.M., Ji, X., Byrd, R.
EMBO J. (2013), 32, 2504-2516, PubMed 23942235 , DOI 10.1038/emboj.2013.174 , Abstract

Related entities 1. gp78 RING bound to Ube2g2:G2BR, entity 1, : 97 entities Detail

Related entities 2. gp78 RING bound to Ube2g2:G2BR, entity 2, : 1 : 5 : 2 : 4 entities Detail

Related entities 3. gp78 RING bound to Ube2g2:G2BR, entity 3, : 4 : 1 : 135 entities Detail

Interaction partners 2. gp78 RING bound to Ube2g2:G2BR, entity 2, : 33 : 1 interactors Detail

Interaction partners 3. gp78 RING bound to Ube2g2:G2BR, entity 3, : 32 : 1 interactors Detail

Experiments performed 11 experiments Detail