BMRB: 18718

Alpha4 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift

Authors

Bonet, R., Campbell, I.D.

Assembly

alpha 4 integrin tail

Entity

1. alpha 4 integrin tail (polymer, Thiol state: not present), 37 monomers, 4322.665 Da Detail

GPLGSKAGFF KRQYKSILQE ENRRDSWSYI NSKSNDD

Formula weight

4322.665 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 25.5 %, Completeness (bb): 62.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	25.5 % (72 of 282)	15.0 % (36 of 240)	85.7 % (36 of 42)
Backbone	62.5 % (70 of 112)	46.1 % (35 of 76)	97.2 % (35 of 36)
Sidechain	1.2 % (2 of 170)	0.6 % (1 of 164)	16.7 % (1 of 6)
Aromatic	8.0 % (2 of 25)	4.2 % (1 of 24)	100.0 % (1 of 1)
Methyl	0.0 % (0 of 9)	0.0 % (0 of 9)

1. alpha 4 integrin

GPLGSKAGFF KRQYKSILQE ENRRDSWSYI NSKSNDD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	alpha 4 integrin	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	DSS	natural abundance		0.1 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

Hide sample codition

Release date

2013-07-24

Citation

Characterization of 14-3-3-ζ Interactions with integrin tails
Bonet, R., Vakonakis, I., Campbell, I.D.
J. Mol. Biol. (2013), 425, 3060-3072, PubMed 23763993 , DOI 10.1016/j.jmb.2013.05.024 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. alpha 4 integrin tail, : 2 : 4 entities Detail

Interaction partners 1. alpha 4 integrin tail, : 17 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail