BMRB: 18719

Beta2 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift

Authors

Bonet, R., Campbell, I.D.

Assembly

beta 2 integrin tail

Entity

1. beta 2 integrin tail (polymer, Thiol state: not present), 48 monomers, 5637.340 Da Detail

GPKALIHLSD LREYRRFEKE KLKSQWNNDN PLFKSATTTV MNPKFAES

Formula weight

5637.34 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.6 %, Completeness: 25.5 %, Completeness (bb): 63.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	25.5 % (94 of 368)	15.8 % (50 of 317)	86.3 % (44 of 51)
Backbone	63.3 % (88 of 139)	47.9 % (45 of 94)	95.6 % (43 of 45)
Sidechain	2.6 % (6 of 229)	2.2 % (5 of 223)	16.7 % (1 of 6)
Aromatic	7.1 % (2 of 28)	3.7 % (1 of 27)	100.0 % (1 of 1)
Methyl	0.0 % (0 of 20)	0.0 % (0 of 20)

1. beta 2 integrin

GPKALIHLSD LREYRRFEKE KLKSQWNNDN PLFKSATTTV MNPKFAES

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	beta 2 integrin	[U-100% 13C; U-100% 15N]		0.6 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		50 mM
4	DSS	natural abundance		0.1 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

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Release date

2013-07-24

Citation

Characterization of 14-3-3-ζ Interactions with integrin tails
Bonet, R., Vakonakis, I., Campbell, I.D.
J. Mol. Biol. (2013), 425, 3060-3072, PubMed 23763993 , DOI 10.1016/j.jmb.2013.05.024 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. beta 2 integrin tail, : 6 : 64 entities Detail

Interaction partners 1. beta 2 integrin tail, : 23 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail