Solution Studies of Staphylococcal Nuclease H124L. 2. 1H, 13C, and 15N Chemical Shift Assignments for the Unligated Enzyme and Analysis of Chemical Shift Changes That Accompany Formation of the Nuclease-Thymidine 3',5'-Bisphosphate-Calcium
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 15.0 % (137 of 912) | 15.0 % (137 of 912) |
Backbone | 40.5 % (117 of 289) | 40.5 % (117 of 289) |
Sidechain | 3.2 % (20 of 623) | 3.2 % (20 of 623) |
Aromatic | 0.0 % (0 of 55) | 0.0 % (0 of 55) |
Methyl | 2.7 % (2 of 75) | 2.7 % (2 of 75) |
1. micrococcal nuclease
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SEDTemperature 318 K, pH 5.1