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Solution Studies of Staphylococcal Nuclease H124L. 2. 1H, 13C, and 15N Chemical Shift Assignments for the Unligated Enzyme and Analysis of Chemical Shift Changes That Accompany Formation of the Nuclease-Thymidine 3',5'-Bisphosphate-Calcium
Authors
Wang, J., Hinck, A.P., Loh, S.N., LeMaster, D.M., Markley, J.L.
Assembly
micrococcal nuclease
Entity
1. micrococcal nuclease (polymer), 143 monomers, 16214.55 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED


Formula weight
16214.55 Da
Source organism
Staphylococcus aureus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 78.3 %, Completeness: 12.3 %, Completeness (bb): 37.5 % Detail

Polymer type: polypeptide(L)

Total1H
All12.3 % (112 of 912)12.3 % (112 of 912)
Backbone37.5 % (105 of 280)37.5 % (105 of 280)
Sidechain 1.1 % (7 of 632) 1.1 % (7 of 632)
Aromatic 0.0 % (0 of 55) 0.0 % (0 of 55)
Methyl 0.0 % (0 of 75) 0.0 % (0 of 75)

1. micrococcal nuclease

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED

Sample

Temperature 318 K, pH 5.1



Release date
1995-07-30
Citation
Solution studies of staphylococcal nuclease H124L. 2. 1H, 13C, and 15N chemical shift assignments for the unligated enzyme and analysis of chemical shift changes that accompany formation of the nuclease-thymidine 3',5'-bisphosphate-calcium ternary complex
Wang, J., Hinck, A.P., Loh, S.N., LeMaster, D.M., Markley, J.L.
Biochemistry (1992), 31, 921-936, PubMed 1731949 , DOI 10.1021/bi00118a039 ,
Entries sharing articles BMRB: 4 entries Detail
  BMRB: 1875 released on 1995-07-30
    Title Solution Studies of Staphylococcal Nuclease H124L. 2. 1H, 13C, and 15N Chemical Shift Assignments for the Unligated Enzyme and Analysis of Chemical Shift Changes That Accompany Formation of the Nuclease-Thymidine 3',5'-Bisphosphate-Calcium
  BMRB: 1876 released on 1995-07-30
    Title Solution Studies of Staphylococcal Nuclease H124L. 2. 1H, 13C, and 15N Chemical Shift Assignments for the Unligated Enzyme and Analysis of Chemical Shift Changes That Accompany Formation of the Nuclease-Thymidine 3',5'-Bisphosphate-Calcium
  BMRB: 1877 released on 1995-07-30
    Title Solution Studies of Staphylococcal Nuclease H124L. 2. 1H, 13C, and 15N Chemical Shift Assignments for the Unligated Enzyme and Analysis of Chemical Shift Changes That Accompany Formation of the Nuclease-Thymidine 3',5'-Bisphosphate-Calcium
  BMRB: 1878 released on 1995-07-30
    Title Solution Studies of Staphylococcal Nuclease H124L. 2. 1H, 13C, and 15N Chemical Shift Assignments for the Unligated Enzyme and Analysis of Chemical Shift Changes That Accompany Formation of the Nuclease-Thymidine 3',5'-Bisphosphate-Calcium
Related entities 1. micrococcal nuclease, : 1 : 7 : 43 : 73 entities Detail
Experiments performed 1 experiments Detail