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Solution Studies of Staphylococcal Nuclease H124L. 2. 1H, 13C, and 15N Chemical Shift Assignments for the Unligated Enzyme and Analysis of Chemical Shift Changes That Accompany Formation of the Nuclease-Thymidine 3',5'-Bisphosphate-Calcium
Authors
Wang, J., Hinck, A.P., Loh, S.N., LeMaster, D.M., Markley, J.L.
Assembly
micrococcal nuclease
Entity
1. micrococcal nuclease (polymer), 143 monomers, 16214.55 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED


Formula weight
16214.55 Da
Source organism
Staphylococcus aureus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 90.9 %, Completeness: 40.4 %, Completeness (bb): 30.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All40.4 % (700 of 1734)48.9 % (446 of 912)37.7 % (252 of 669) 1.3 % (2 of 153)
Backbone30.9 % (259 of 837)43.9 % (123 of 280)31.9 % (134 of 420) 1.5 % (2 of 137)
Sidechain45.4 % (468 of 1031)51.1 % (323 of 632)37.9 % (145 of 383) 0.0 % (0 of 16)
Aromatic96.4 % (106 of 110)98.2 % (54 of 55)96.3 % (52 of 54) 0.0 % (0 of 1)
Methyl90.0 % (135 of 150)96.0 % (72 of 75)84.0 % (63 of 75)

1. micrococcal nuclease

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED

Sample

Temperature 318 K, pH 5.5



LACS Plot; CA
Referencing offset: 1.49 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 1.49 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.11 ppm, Outliers: 1 Detail
Release date
1995-07-30
Citation
Solution studies of staphylococcal nuclease H124L. 2. 1H, 13C, and 15N chemical shift assignments for the unligated enzyme and analysis of chemical shift changes that accompany formation of the nuclease-thymidine 3',5'-bisphosphate-calcium ternary complex
Wang, J., Hinck, A.P., Loh, S.N., LeMaster, D.M., Markley, J.L.
Biochemistry (1992), 31, 921-936, PubMed 1731949 , DOI 10.1021/bi00118a039 ,
Related entities 1. micrococcal nuclease, : 1 : 7 : 43 : 73 entities Detail
Experiments performed 1 experiments Detail