Solution Studies of Staphylococcal Nuclease H124L. 2. 1H, 13C, and 15N Chemical Shift Assignments for the Unligated Enzyme and Analysis of Chemical Shift Changes That Accompany Formation of the Nuclease-Thymidine 3',5'-Bisphosphate-Calcium
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 44.7 % (773 of 1729) | 53.4 % (487 of 912) | 41.0 % (274 of 669) | 8.1 % (12 of 148) |
Backbone | 35.7 % (302 of 846) | 47.8 % (138 of 289) | 36.2 % (152 of 420) | 8.8 % (12 of 137) |
Sidechain | 50.0 % (509 of 1017) | 56.0 % (349 of 623) | 41.8 % (160 of 383) | 0.0 % (0 of 11) |
Aromatic | 96.4 % (106 of 110) | 98.2 % (54 of 55) | 96.3 % (52 of 54) | 0.0 % (0 of 1) |
Methyl | 91.3 % (137 of 150) | 97.3 % (73 of 75) | 85.3 % (64 of 75) |
1. micrococcal nuclease
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SEDTemperature 318 K, pH 5.5