Solution Studies of Staphylococcal Nuclease H124L. 2. 1H, 13C, and 15N Chemical Shift Assignments for the Unligated Enzyme and Analysis of Chemical Shift Changes That Accompany Formation of the Nuclease-Thymidine 3',5'-Bisphosphate-Calcium
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED
Polymer type: polypeptide(L)
Total | 15N | |
---|---|---|
All | 81.1 % (120 of 148) | 81.1 % (120 of 148) |
Backbone | 87.6 % (120 of 137) | 87.6 % (120 of 137) |
Sidechain | 0.0 % (0 of 11) | 0.0 % (0 of 11) |
Aromatic | 0.0 % (0 of 1) | 0.0 % (0 of 1) |
1. micrococcal nuclease
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SEDTemperature 318 K, pH 5.5