BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	93.8 % (1085 of 1157)	91.9 % (546 of 594)	96.3 % (439 of 456)	93.5 % (100 of 107)
Backbone	93.7 % (590 of 630)	92.4 % (206 of 223)	94.4 % (287 of 304)	94.2 % (97 of 103)
Sidechain	93.1 % (576 of 619)	90.3 % (335 of 371)	97.5 % (238 of 244)	75.0 % (3 of 4)
Aromatic	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (41 of 41)	100.0 % (1 of 1)
Methyl	97.5 % (117 of 120)	95.0 % (57 of 60)	100.0 % (60 of 60)

1. Ubl protein HVO 2177

GGGRDYKDDD DKGTMELELR FFATFREVVG QKSIYWRVDD DATVGDVLRS LEAEYDGLAG RLIEDGEVKP HVNVLKNGRE VVHLDGMATA LDDGDAVSVF PPVAGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
2	NaCl	natural abundance	500 mM
3	Na-K phosphate	natural abundance	25 mM
4	EDTA	natural abundance	0.2 mM
5	NaN3	natural abundance	0.02 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
7	NaCl	natural abundance	500 mM
8	Na-K phosphate	natural abundance	25 mM
9	EDTA	natural abundance	0.2 mM
10	NaN3	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.772 ppm	na	indirect	0.2514495
¹H	water	protons	4.772 ppm	internal	direct	1.0
¹⁵N	water	protons	4.772 ppm	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.772 ppm	na	indirect	0.2514495
¹H	water	protons	4.772 ppm	internal	direct	1.0
¹⁵N	water	protons	4.772 ppm	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.772 ppm	na	indirect	0.2514495
¹H	water	protons	4.772 ppm	internal	direct	1.0
¹⁵N	water	protons	4.772 ppm	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.772 ppm	na	indirect	0.2514495
¹H	water	protons	4.772 ppm	internal	direct	1.0
¹⁵N	water	protons	4.772 ppm	na	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M19, Strand ID: A Detail

Release date

2013-08-04

Citation

Crystal structure of the ubiquitin-like small archaeal modifier protein 2 from Haloferax volcanii
Li, Y., Maciejewski, M.W., Martin, J., Jin, K., Zhang, Y., Maupin-Furlow, J.A., Hao, B.
Protein Sci. (2013), 22, 1206-1217, PubMed 23821306 , DOI 10.1002/pro.2305 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: D4GVB0 released on 2014-05-14
    Title SAMP3_HALVD Entity Small archaeal modifier protein 3
  Swiss-Prot: D4GZE7 released on 2010-08-10
    Title SAMP2_HALVD Entity Small archaeal modifier protein 2

Related entities 1. Haloferax volcanii HVO 2177 protein, : 1 : 1 : 43 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
2	NaCl	natural abundance	500 mM
3	Na-K phosphate	natural abundance	25 mM
4	EDTA	natural abundance	0.2 mM
5	NaN3	natural abundance	0.02 %

2 2D 1H-13C HSQC

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
7	NaCl	natural abundance	500 mM
8	Na-K phosphate	natural abundance	25 mM
9	EDTA	natural abundance	0.2 mM
10	NaN3	natural abundance	0.02 %

3 3D HNCACB

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
2	NaCl	natural abundance	500 mM
3	Na-K phosphate	natural abundance	25 mM
4	EDTA	natural abundance	0.2 mM
5	NaN3	natural abundance	0.02 %

4 3D HNCO

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
2	NaCl	natural abundance	500 mM
3	Na-K phosphate	natural abundance	25 mM
4	EDTA	natural abundance	0.2 mM
5	NaN3	natural abundance	0.02 %

5 3D HCACO

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
2	NaCl	natural abundance	500 mM
3	Na-K phosphate	natural abundance	25 mM
4	EDTA	natural abundance	0.2 mM
5	NaN3	natural abundance	0.02 %

6 3D HBHA(CO)NH

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
2	NaCl	natural abundance	500 mM
3	Na-K phosphate	natural abundance	25 mM
4	EDTA	natural abundance	0.2 mM
5	NaN3	natural abundance	0.02 %

7 3D C(CO)NH

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
2	NaCl	natural abundance	500 mM
3	Na-K phosphate	natural abundance	25 mM
4	EDTA	natural abundance	0.2 mM
5	NaN3	natural abundance	0.02 %

8 3D H(CCO)NH

Instrument

Varian VNMRS - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
2	NaCl	natural abundance	500 mM
3	Na-K phosphate	natural abundance	25 mM
4	EDTA	natural abundance	0.2 mM
5	NaN3	natural abundance	0.02 %

9 3D HCCH-TOCSY

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
7	NaCl	natural abundance	500 mM
8	Na-K phosphate	natural abundance	25 mM
9	EDTA	natural abundance	0.2 mM
10	NaN3	natural abundance	0.02 %

10 2D 1H-13C HSQC aromatic

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
7	NaCl	natural abundance	500 mM
8	Na-K phosphate	natural abundance	25 mM
9	EDTA	natural abundance	0.2 mM
10	NaN3	natural abundance	0.02 %

11 2D 1H-1H NOESY

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
7	NaCl	natural abundance	500 mM
8	Na-K phosphate	natural abundance	25 mM
9	EDTA	natural abundance	0.2 mM
10	NaN3	natural abundance	0.02 %

12 3D 1H-15N NOESY

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
2	NaCl	natural abundance	500 mM
3	Na-K phosphate	natural abundance	25 mM
4	EDTA	natural abundance	0.2 mM
5	NaN3	natural abundance	0.02 %

13 3D 1H-13C NOESY aliphatic

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
7	NaCl	natural abundance	500 mM
8	Na-K phosphate	natural abundance	25 mM
9	EDTA	natural abundance	0.2 mM
10	NaN3	natural abundance	0.02 %

14 3D 1H-13C NOESY aromatic

Instrument

Varian VNMRS - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	Ubl protein HVO_2177	[U-99% 13C; U-99% 15N]	1 mM
7	NaCl	natural abundance	500 mM
8	Na-K phosphate	natural abundance	25 mM
9	EDTA	natural abundance	0.2 mM
10	NaN3	natural abundance	0.02 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_18850_2m19.nef
Input source #2: Coordindates	2m19.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

----------------------10--------20--------30--------40--------50--------60--------70--------80------
GGGRDYKDDDDKGTMELELRFFATFREVVGQKSIYWRVDDDATVGDVLRSLEAEYDGLAGRLIEDGEVKPHVNVLKNGREVVHLDGMATALDDGDAVSVF
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GGGRDYKDDDDKGTMELELRFFATFREVVGQKSIYWRVDDDATVGDVLRSLEAEYDGLAGRLIEDGEVKPHVNVLKNGREVVHLDGMATALDDGDAVSVF
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

--90--
PPVAGG
||||||
PPVAGG
------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	106	0	0	100.0

Content subtype: combined_18850_2m19.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1093		96.2 (chain: A, length: 106)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	4462 (1)	noe	92.5 (chain: A, length: 106)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	164 (164)	.	86.8 (chain: A, length: 106)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 106, Sequence coverage: 96.2%
- Total number of assignments
  - ¹H chemical shifts: 554
  - ¹³C chemical shifts: 441
  - ¹⁵N chemical shifts: 98
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	594	554	93.3
¹³C chemical shifts	456	441	96.7
¹⁵N chemical shifts	114	98	86.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	223	207	92.8
¹³C chemical shifts	212	200	94.3
¹⁵N chemical shifts	103	95	92.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	371	347	93.5
¹³C chemical shifts	244	241	98.8
¹⁵N chemical shifts	11	3	27.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	62	62	100.0
¹³C chemical shifts	62	62	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	42	42	100.0
¹³C chemical shifts	41	41	100.0
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 106, Sequence coverage: 92.5%
- Total number of restraints: 4453
- Weight of restraints: 1.0 (4453)
- Potential type of restraints: square-well-parabolic (4453)
- Range of distance target values: 2.65, 3.9 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 106, Sequence coverage: 86.8%
- Total number of restraints: 164
- Total number of restraints per polymer type: 164
- Weight of restraints: 1.0 (164)
- Potential type of restraints: square-well-parabolic (164)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords ubiquitin-like protein

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Found 1 Document (0.932 seconds)

BMRB: 18850

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. Haloferax volcanii HVO 2177 protein, : 1 : 1 : 43 entities Detail

Experiments performed 14 experiments Detail

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NMR combined restraints 4 contents Detail