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ASFV Pol X structure
Authors
Wu, W., Su, M., Tsai, M.
Assembly
ASFV Pol X
Entity
1. ASFV Pol X (polymer, Thiol state: all free), 174 monomers, 20314.16 Da Detail

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL


Formula weight
20314.16 Da
Source organism
African swine fever virus
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 88.5 %, Completeness: 37.4 %, Completeness (bb): 72.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All37.4 % (841 of 2248)19.7 % (232 of 1179)51.8 % (454 of 877)80.7 % (155 of 192)
Backbone72.8 % (745 of 1024)46.5 % (159 of 342)84.0 % (432 of 514)91.7 % (154 of 168)
Sidechain17.4 % (242 of 1390) 8.7 % (73 of 837)31.8 % (168 of 529) 4.2 % (1 of 24)
Aromatic 3.8 % (6 of 160) 5.0 % (4 of 80) 2.5 % (2 of 79) 0.0 % (0 of 1)
Methyl 8.5 % (21 of 246) 5.7 % (7 of 123)11.4 % (14 of 123)

1. entity

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5


#NameIsotope labelingTypeConcentration
1entity[U-100% 13C; U-100% 15N; U-80% 2H]1.0 mM
2DTT[U-2H]10 mM
3potassium chloridenatural abundance50 mM
4boratenatural abundance50 mM
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5


#NameIsotope labelingTypeConcentration
5entity[U-100% 13C; U-100% 15N]1.0 mM
6DTT[U-2H]10 mM
7potassium chloridenatural abundance50 mM
8boratenatural abundance50 mM

LACS Plot; CA
Referencing offset: 0.69 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.69 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.43 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2M2T, Strand ID: A Detail


Release date
2014-03-30
Citation
How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation
Wu, W., Su, M., Wu, J., Kumar, S., Lim, L., Wang, C., Nelissen, F.H.T., Chen, M., Doreleijers, J.F., Wijmenga, S.S., Tsai, M.
J. Am. Chem. Soc. (2014), 136, 4927-4937, PubMed 24617852 , DOI 10.1021/ja4102375 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 18934 released on 2014-03-30
    Title Binary complex of African Swine Fever Virus Pol X with MgdGTP
  BMRB: 18935 released on 2014-03-30
    Title African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA
Related entities 1. ASFV Pol X, : 1 : 14 : 2 : 3 : 95 entities Detail
Experiments performed 8 experiments Detail
Keywords DNA polymerase, NMR