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African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA
Authors
Wu, W., Su, M., Tsai, M.
Assembly
African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA
Entity
1. African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA, entity (polymer, Thiol state: all free), 174 monomers, 20314.16 Da Detail

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL


2. African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA, entity MG (non-polymer), 24.305 × 2 Da
3. African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA, entity DGT (non-polymer), 507.181 Da
4. DNA (polymer, Thiol state: not present), 23 monomers, 7204.571 Da Detail

GGCGAAGCCG GGTGCGAAGC ACC


Total weight
28074.521 Da
Max. entity weight
20314.16 Da
Source organism
African swine fever virus
Exptl. method
SOLUTION NMR
Refine. method
distance geometry
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 84.3 %, Completeness: 44.4 %, Completeness (bb): 68.5 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

Total1H13C15N
All44.4 % (1097 of 2470)24.8 % (348 of 1401)66.4 % (582 of 877)87.0 % (167 of 192)
Backbone68.5 % (812 of 1185)33.2 % (167 of 503)93.2 % (479 of 514)98.8 % (166 of 168)
Sidechain30.5 % (442 of 1451)20.3 % (182 of 898)49.0 % (259 of 529) 4.2 % (1 of 24)
Aromatic12.1 % (25 of 206)19.8 % (25 of 126) 0.0 % (0 of 79) 0.0 % (0 of 1)
Methyl73.3 % (181 of 247)69.4 % (86 of 124)77.2 % (95 of 123)

1. entity

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL

4. DNA

GGCGAAGCCG GGTGCGAAGC ACC

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5


#NameIsotope labelingTypeConcentration
1African Swine Fever Virus Pol XILV-CH3-[U-13C; U-15N; U-2H]0.5 mM
2Mgnatural abundance10 mM
3dGTPnatural abundance4 mM
4DNA[U-100% 13C; U-100% 15N]0.6 mM
5Boratenatural abundance50 mM
6DTT[U-2H]10 mM
7potassium chloridenatural abundance200 mM

LACS Plot; CA
Referencing offset: 0.77 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.77 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.54 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2M2V, Strand ID: A Detail


Release date
2014-03-30
Citation
How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation
Wu, W., Su, M., Wu, J., Kumar, S., Lim, L., Wang, C., Nelissen, F.H.T., Chen, M., Doreleijers, J.F., Wijmenga, S.S., Tsai, M.
J. Am. Chem. Soc. (2014), 136, 4927-4937, PubMed 24617852 , DOI 10.1021/ja4102375 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 18933 released on 2014-03-30
    Title ASFV Pol X structure
  BMRB: 18934 released on 2014-03-30
    Title Binary complex of African Swine Fever Virus Pol X with MgdGTP
Related entities 1. African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA, entity, : 1 : 14 : 2 : 3 : 95 entities Detail
Experiments performed 10 experiments Detail
Keywords DNA polymerase, NMR