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BMRB: 18935

2M2V

African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA

Authors

Wu, W., Su, M., Tsai, M.

Assembly

African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA

Entity

1. African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA, entity (polymer, Thiol state: all free), 174 monomers, 20314.16 Da Detail

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL

2. African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA, entity MG (non-polymer), 24.305 × 2 Da
3. African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA, entity DGT (non-polymer), 507.181 Da
4. DNA (polymer, Thiol state: not present), 23 monomers, 7204.571 Da Detail

GGCGAAGCCG GGTGCGAAGC ACC

Total weight

28074.521 Da

Max. entity weight

20314.16 Da

Source organism

African swine fever virus

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.3 %, Completeness: 46.0 %, Completeness (bb): 70.2 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	46.0 % (1131 of 2460)	26.6 % (372 of 1401)	67.7 % (594 of 877)	90.7 % (165 of 182)
Backbone	70.2 % (837 of 1193)	36.4 % (186 of 511)	94.6 % (486 of 514)	98.2 % (165 of 168)
Sidechain	31.5 % (452 of 1433)	20.9 % (186 of 890)	50.3 % (266 of 529)	0.0 % (0 of 14)
Aromatic	15.5 % (32 of 206)	22.2 % (28 of 126)	5.1 % (4 of 79)	0.0 % (0 of 1)
Methyl	76.5 % (189 of 247)	73.4 % (91 of 124)	79.7 % (98 of 123)

1. entity

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL

4. DNA

GGCGAAGCCG GGTGCGAAGC ACC

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	African Swine Fever Virus Pol X	ILV-CH3-[U-13C; U-15N; U-2H]	0.5 mM
2	Mg	natural abundance	10 mM
3	dGTP	natural abundance	4 mM
4	DNA	[U-100% 13C; U-100% 15N]	0.6 mM
5	Borate	natural abundance	50 mM
6	DTT	[U-2H]	10 mM
7	potassium chloride	natural abundance	200 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.77 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.77 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.54 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M2V, Strand ID: A Detail

Release date

2014-03-30

Citation

How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation
Wu, W., Su, M., Wu, J., Kumar, S., Lim, L., Wang, C., Nelissen, F.H.T., Chen, M., Doreleijers, J.F., Wijmenga, S.S., Tsai, M.
J. Am. Chem. Soc. (2014), 136, 4927-4937, PubMed 24617852 , DOI 10.1021/ja4102375 , Abstract

Related entities 1. African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA, entity, : 1 : 20 : 2 : 3 : 104 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC