BMRB: 18948

STRUCTURE OF LASSO PEPTIDE ASTEXIN-1(19)

Authors

Zimmermann, M., Hegemann, J.D., Xie, X., Marahiel, M.A.

Assembly

LASSO PEPTIDE ASTEXIN-1(19)

Entity

1. LASSO PEPTIDE ASTEXIN-1(19) (polymer, Thiol state: not present), 19 monomers, 2112.210 Da Detail

GLSQGVEPDI GQTYFEESR

Formula weight

2112.21 Da

Source organism

Asticcacaulis excentricus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.1 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.1 % (110 of 111)	99.1 % (110 of 111)
Backbone	100.0 % (40 of 40)	100.0 % (40 of 40)
Sidechain	98.6 % (70 of 71)	98.6 % (70 of 71)
Aromatic	88.9 % (8 of 9)	88.9 % (8 of 9)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. ASTEXIN-1(19)

GLSQGVEPDI GQTYFEESR

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	ASTEXIN-1(19)	natural abundance		5.7 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

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Calculated from 20 models in PDB: 2M37, Strand ID: A Detail

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Release date

2014-02-13

Citation

The astexin-1 lasso peptides: biosynthesis, stability, and structural studies
Zimmermann, M., Hegemann, J.D., Xie, X., Marahiel, M.A.
Chem. Biol. (2013), 20, 558-569, PubMed 23601645 , DOI 10.1016/j.chembiol.2013.03.013 , Abstract

Related entities 1. LASSO PEPTIDE ASTEXIN-1(19), : 1 : 2 : 16 entities Detail

Experiments performed 3 experiments Detail