Analysis of the structural and molecular basis of voltage-sensitive sodium channel inhibition by the spider toxin, Huwentoxin-IV ( -TRTX-Hh2a).
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 74.4 % (163 of 219) | 74.4 % (163 of 219) |
Backbone | 94.2 % (65 of 69) | 94.2 % (65 of 69) |
Sidechain | 65.3 % (98 of 150) | 65.3 % (98 of 150) |
Aromatic | 6.7 % (1 of 15) | 6.7 % (1 of 15) |
Methyl | 100.0 % (13 of 13) | 100.0 % (13 of 13) |
1. Huwentoxin-IV
ECLEIFKACN PSNDQCCKSS KLVCSRKTRW CAYQISolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Huwentoxin-IV | natural abundance | 2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium azide | natural abundance | 150 uM | |
4 | EDTA | [U-99% 2H] | 100 uM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |